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首页> 外文期刊>The European Journal of Neuroscience >Quantitative effects produced by modifications of neuronal activity on the size of GABA receptor clusters in hippocampal slice cultures.
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Quantitative effects produced by modifications of neuronal activity on the size of GABA receptor clusters in hippocampal slice cultures.

机译：在海马切片培养物中，神经元活性的改变对GABA受体簇大小的影响。

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Abstract The number and strength of GABAergic synapses needs to be precisely adjusted for adequate control of excitatory activity. We investigated to what extent the size of GABA(A) receptor clusters at inhibitory synapses is under the regulation of neuronal activity. Slices from P7 rat hippocampus were cultured for 13 days in the presence of bicuculline or 4-aminopyridine (4-AP) to increase neuronal activity, or DNQX to decrease activity. The changes provoked by these treatments on clusters immunoreactive for the alpha1 and alpha2 subunits of the GABA(A) receptor or gephyrin were quantitatively evaluated. While an increase in activity augmented the density of these clusters, a decrease in activity provoked, in contrast, a decrease in their density. An inverse regulation was observed for the size of individual clusters. Bicuculline and 4-AP decreased whilst DNQX increased the mean size of the clusters. When the pharmacological treatments were applied for 2 days instead of 2 weeks, no effects on the size of the clusters were observed. The variations in the mean size of individual clusters were mainly due to changes in the number of small clusters. Finally, a regulation of the size of GABA(A) receptor clusters occurred during development in vivo, with a decrease of the mean size of the clusters between P7 and P21. This physiological change was also the result of an increase in the number of small clusters. These results indicate that neuronal activity regulates the mean size of GABA(A) receptor- and gephyrin-immunoreactive clusters by modifying specifically the number of synapses with small clusters of receptors.
机译：摘要为精确控制兴奋性，需要精确调节GABA能突触的数量和强度。我们调查了神经突触调控下，在抑制突触中GABA（A）受体簇的大小。将P7大鼠海马的切片在双小分子或4-氨基吡啶（4-AP）的存在下培养13天，以增加神经元活性，或DNQX减少活性。定量评估了这些处理对GABA（A）受体或gephyrin的alpha1和alpha2亚基具有免疫反应性的簇引起的变化。活性的增加虽然增加了这些簇的密度，但是相反，活性的降低却引起了它们密度的降低。观察到单个簇大小的反调节。 Bicuculline和4-AP减少，而DNQX增加了簇的平均大小。当应用药理治疗2天而不是2周时，未观察到对簇的大小的影响。单个簇的平均大小的变化主要是由于小簇数目的变化。最后，体内发育过程中发生了GABA（A）受体簇大小的调节，P7和P21之间簇的平均大小减小。这种生理变化也是小簇数目增加的结果。这些结果表明，神经元活性通过特定地修饰具有小簇受体的突触的数量来调节GABA（A）受体和gephyrin免疫反应簇的平均大小。

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