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首页> 外文期刊>The European Journal of Neuroscience >Synthesis, localization and externalization of galectin-1 in mature dorsal root ganglion neurons and Schwann cells.
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Synthesis, localization and externalization of galectin-1 in mature dorsal root ganglion neurons and Schwann cells.

机译：galectin-1在成熟背根神经节神经元和雪旺氏细胞中的合成，定位和外化。

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摘要

We recently confirmed that oxidized galectin-1 is a novel factor enhancing axonal growth in peripheral nerves after axotomy, but the process of extracellular release and oxidization of endogenous galectin-1 in the injured nervous tissue remains unknown. In the present study, we examined the distribution of galectin-1 in adult rat dorsal root ganglia (DRG) in vivo and in vitro. By RT-PCR analysis and in situ hybridization histochemistry, galectin-1 mRNA was detected in both DRG neurons and non-neuronal cells. Immunohistochemical analyses revealed that galectin-1 was distributed diffusely throughout the cytoplasm in smaller diameter neurons and Schwann cells in DRG sections. In contrast, the immunoreactivity for galectin-1 was detected in almost all DRG neurons from an early stage in culture (3 h after seeding) and was restricted to the surface and/or extracellular region of neurons and Schwann cells at later stages in culture. In a manner similar to the primary cultured cells, we also observed the surface and extracellular expression of this molecule in immortalized adult mouse Schwann cells (IMS32). Western blot analysis has revealed that both reduced and oxidized forms of galectin-1 were detected in culture media of DRG neurons and IMS32. These findings suggest that galectin-1 is externalized from DRG neurons and Schwann cells upon axonal injury. Some of the molecules in the extracellular milieu may be converted to the oxidized form, which lacks lectin activity but could act on neural tissue as a cytokine.
机译：我们最近证实，氧化型半乳糖凝集素-1是一种新的增强轴突切开术后周围神经轴突生长的因子，但是在受伤的神经组织中细胞外释放和内源性半乳糖凝集素-1的氧化过程仍然未知。在本研究中，我们研究了galectin-1在体内和体外在成年大鼠背根神经节（DRG）中的分布。通过RT-PCR分析和原位杂交组织化学，在DRG神经元和非神经元细胞中均检测到galectin-1 mRNA。免疫组织化学分析显示，galectin-1分散分布在DRG切片中较小直径神经元和Schwann细胞的整个细胞质中。相反，从培养的早期（接种后3小时）开始，几乎所有DRG神经元都检测到了galectin-1的免疫反应性，并在培养的后期局限于神经元和雪旺氏细胞的表面和/或细胞外区域。以与原代培养细胞相似的方式，我们还观察了该分子在永生化成年小鼠雪旺细胞（IMS32）中的表面和细胞外表达。蛋白质印迹分析表明，在DRG神经元和IMS32的培养基中均检测到了galectin-1的还原和氧化形式。这些发现表明，轴突损伤后，galectin-1从DRG神经元和雪旺氏细胞外部化。细胞外环境中的某些分子可能会转化为氧化形式，这种形式缺乏凝集素活性，但可能作为细胞因子作用于神经组织。

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