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首页> 外文期刊>The European Journal of Neuroscience >Efficacy of doublecortin as a marker to analyse the absolute number and dendritic growth of newly generated neurons in the adult dentate gyrus.
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Efficacy of doublecortin as a marker to analyse the absolute number and dendritic growth of newly generated neurons in the adult dentate gyrus.

机译：Doublecortin作为标记物分析成年齿状回中新生成的神经元的绝对数量和树突生长的功效。

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Doublecortin (DCX), a microtubule-associated phosphoprotein, has been recently utilized as a marker of newly born neurons in the adult dentate gyrus (DG). Nonetheless, it is unknown whether DCX exclusively labels newly formed neurons, as certain granule cells with the phenotype of differentiated neurons express DCX. We addressed the authenticity of DCX as a marker of new neurons in the adult DG by quantifying cells that are positive for 5'-bromodeoxyuridine (BrdU), DCX and both BrdU and DCX in hippocampal tissues of adult rats treated with daily injections of BrdU for 12 consecutive days. We provide new evidence that neurons visualized with DCX immunostaining in the adult rat DG are new neurons that are predominantly born during the 12 days before euthanasia. This is confirmed by the robust expression of BrdU in 90% of DCX-positive neurons in the DG of animals injected with BrdU for 12 days. Furthermore, DCX expression is specific to newly generated healthy neurons, as virtually all DCX-positive cells express early neuronal antigens but lack antigens specific to glia, undifferentiated cells or apoptotic cells. As DCX expression is also robust in the dendrites, DCX immunocytochemistry of thicker sections facilitates quantification of the dendritic growth in newly born neurons. Thus, both absolute number and dendritic growth of new neurons that are generated in the adult DG over a 12-day period can be quantified reliably with DCX immunostaining. This could be particularly useful for analysing changes in dentate neurogenesis in human hippocampal tissues as a function of ageing or neurodegenerative diseases.
机译：双肾上腺皮质激素（DCX）是一种与微管相关的磷蛋白，最近已被用作成人齿状回（DG）中新生神经元的标志物。但是，未知的是DCX是否专门标记新形成的神经元，因为某些具有分化神经元表型的颗粒细胞表达DCX。我们通过定量每天注射BrdU的成年大鼠海马组织中5'-溴脱氧尿苷（BrdU），DCX以及BrdU和DCX阳性的细胞来解决DCX作为成年DG中新神经元标记的真实性连续12天。我们提供了新的证据，在成年大鼠DG中以DCX免疫染色可视化的神经元是主要在安乐死之前的12天内出生的新神经元。通过注射BrdU的动物DG中90％的DCX阳性神经元中BrdU的强劲表达，可以证实这一点。此外，DCX表达对新生健康神经元具有特异性，因为几乎所有DCX阳性细胞均表达早期神经元抗原，但缺乏对神经胶质，未分化细胞或凋亡细胞具有特异性的抗原。由于DCX在树突中的表达也很稳定，因此较厚切片的DCX免疫细胞化学有助于量化新生神经元中树突的生长。因此，可以用DCX免疫染色法可靠地量化在12天的成人DG中生成的新神经元的绝对数量和树突状生长。这对于分析人类海马组织中齿状神经发生的变化与衰老或神经退行性疾病的关系特别有用。

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