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首页> 外文期刊>The European Journal of Neuroscience >Ca signalling and gap junction coupling within and between pigment epithelium and neural retina in the developing chick.
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Ca signalling and gap junction coupling within and between pigment epithelium and neural retina in the developing chick.

机译：发育中雏鸡的色素上皮细胞和神经视网膜之间以及它们之间的Ca信号传导和间隙连接偶联。

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Abstract Development of the neural retina is controlled in part by the adjacent retinal pigment epithelium (RPE). To understand better the mechanisms involved, we investigated calcium signalling and gap junctional coupling within and between the RPE and the neural retina in embryonic day (E) 5 chick. We show that the RPE and the ventricular zone (VZ) of the neural retina display spontaneous Ca(2+) transients. In the RPE, these often spread as waves between neighbouring cells. In the VZ, the frequency of both Ca(2+) transients and waves was lower than in RPE, but increased two-fold in its presence. Ca(2+) signals occasionally crossed the boundary between the RPE and VZ in either direction. In both tissues, the frequency of propagating Ca(2+) waves, but not of individual cell transients, was reduced by gap junction blockers. Use of the gap junction permeant tracer Neurobiotin showed that neural retina cells are coupled into clusters that span the thickness of the retina, and that RPE cells are both coupled together and to clusters of cells in the neural retina. Immunolabelling for Cx43 showed this gap junction protein is present at the junction between the RPE and VZ and thus could potentially mediate the coupling of the two tissues. Immunolabelling for beta-tubulin and vimentin showed that clusters of coupled cells in the neural retina comprised mainly progenitor cells. We conclude that gap junctions between progenitor cells, and between these cells and the RPE, may orchestrate retinal proliferation/differentiation, via the propagation of Ca(2+) or other signalling molecules.
机译：摘要神经视网膜的发育部分受邻近视网膜色素上皮（RPE）的控制。为了更好地理解所涉及的机制，我们调查了在胚胎第5天（R）雏鸡中RPE与神经视网膜之间以及之间的钙信号传导和间隙连接偶联。我们显示RPE和神经视网膜的心室区（VZ）显示自发的Ca（2+）瞬变。在RPE中，这些通常以波的形式在相邻小区之间传播。在VZ中，Ca（2+）瞬变和波的频率均低于RPE，但在存在时增加了两倍。 Ca（2+）信号有时会沿任一方向越过RPE和VZ之间的边界。在这两个组织中，传播Ca（2+）波的频率，但不是单个细胞瞬变的频率，被间隙连接阻滞剂降低了。间隙连接渗透示踪剂Neurobiotin的使用表明，神经视网膜细胞被耦合成跨过视网膜厚度的簇，并且RPE细胞既耦合在一起又耦合到神经视网膜中的细胞簇。 Cx43的免疫标记显示该间隙连接蛋白存在于RPE和VZ之间的连接处，因此可能潜在地介导两个组织的偶联。 β-微管蛋白和波形蛋白的免疫标记显示神经视网膜中耦合细胞簇主要包含祖细胞。我们得出的结论是，祖细胞之间以及这些细胞与RPE之间的间隙连接可能通过Ca（2+）或其他信号分子的传播来协调视网膜增殖/分化。

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