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首页> 外文期刊>The European Journal of Neuroscience >Cilium-attached and excised patch-clamp recordings of odourant-activated Ca-dependent K channels from chemosensory cilia of olfactory receptor neurons.
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Cilium-attached and excised patch-clamp recordings of odourant-activated Ca-dependent K channels from chemosensory cilia of olfactory receptor neurons.

机译：嗅觉受体神经元化学感应纤毛上气味激活的Ca依赖性K通道的Cilium附着和切除的膜片钳记录。

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摘要

It has previously been proposed that a Ca2+-dependent K+ conductance is implicated in the inhibitory odourant response in rat and toad olfactory receptor neurons. Previous whole-cell and single-channel measurements on inside-out excised patches, in addition to immunochemical evidence, indicated the presence of Ca2+-dependent K+ channels in olfactory cilia, the transducing structures of these sensory cells. Ca2+-dependent K+ channels opened in 'on-cilium' membrane patches from C. caudiverbera upon odourant stimulation. Furthermore, after excision in the inside-out configuration, the channel could be opened by micromolar Ca2+, in a Ca2+-dependent fashion, but it was unresponsive to cyclic AMP. We estimated that the Ca2+ concentration in the proximity of a Ca2+-dependent K+ channel within the cilia reaches at least 100 microM during the odour response. The K+ channel displayed a higher selectivity for K+ than for Na+. Our results support a role for this Ca2+-dependent K+ channel in chemotransduction.
机译：先前已经提出，Ca 2+依赖性K +电导与大鼠和蟾蜍嗅觉受体神经元的抑制性气味反应有关。除免疫化学证据外，以前对由内而外切除的贴片进行的全细胞和单通道测量表明，嗅觉纤毛中存在Ca2 +依赖的K +通道，这些感觉细胞的转导结构。 Ca2 +依赖的K +通道在刺激气味后在C. caudiverbera的“纤毛”膜片中打开。此外，以内向外构型切除后，微摩尔Ca2 +可以以Ca2 +依赖的方式打开通道，但它对环状AMP无反应。我们估计，在气味响应过程中，纤毛内Ca2 +依赖性K +通道附近的Ca2 +浓度至少达到100 microM。 K +通道对K +的选择性高于对Na +的选择性。我们的结果支持该依赖Ca2 +的K +通道在化学转导中的作用。

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