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首页> 外文期刊>The European Journal of Neuroscience >The chondroitin sulphate proteoglycan brevican is upregulated by astrocytes after entorhinal cortex lesions in adult rats.
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The chondroitin sulphate proteoglycan brevican is upregulated by astrocytes after entorhinal cortex lesions in adult rats.

机译：成年大鼠内嗅皮质损伤后，星形胶质细胞上调了软骨素硫酸盐蛋白聚糖brevican。

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The chondroitin sulphate proteoglycan brevican is one of the most abundant extracellular matrix molecules in the adult rat brain. It is primarily synthesized by astrocytes and is believed to influence astroglial motility during development and under certain pathological conditions. In order to study a potential role of brevican in the glial reaction after brain injury, its expression was analysed following entorhinal cortex lesion in rats (12 h, 1, 2, 4, 10, 14 and 28 days and 6 months post lesion). In situ hybridization and immunohistochemistry were employed to study brevican mRNA and protein, respectively, in the denervated outer molecular layer of the fascia dentata and at the lesion site. In both regions brevican mRNA was upregulated between 1 and 4 days post lesion. The combination of in situ hybridization with immunohistochemistry for glial fibrillary acidic protein demonstrated that many brevican mRNA-expressing cells are astrocytes. In the denervated zone of the fascia dentata, immunostaining for brevican was increased by 4 days, reached a maximum by 4 weeks and remained detectable up to 6 months post lesion. Electron microscopic immunocytochemistry showed that brevican is a component of the extracellular matrix compartment. At the lesion site a similar time course of brevican upregulation was observed. These data demonstrate that brevican is upregulated in areas of brain damage as well as in areas denervated by a lesion. They suggest a role of brevican in reactive gliosis and are compatible with the hypothesis that brevican is involved in the synaptic reorganization of denervated brain areas.
机译：硫酸软骨素蛋白聚糖brevican是成年大鼠脑中最丰富的细胞外基质分子之一。它主要由星形胶质细胞合成，并被认为在发育过程中和某些病理条件下会影响星形胶质细胞的运动。为了研究布雷维康在脑损伤后神经胶质反应中的潜在作用，分析了大鼠内嗅皮质损伤后（损伤后12 h，1、2、4、10、14、28、28个月和6个月）其表达。原位杂交和免疫组织化学分别用于研究齿状筋膜的失神经外分子层和病变部位的brevican mRNA和蛋白。在两个区域中，在损伤后1至4天之间，brevican mRNA均被上调。胶质纤维酸性蛋白的原位杂交与免疫组织化学的结合表明，许多表达brevican mRNA的细胞都是星形胶质细胞。在齿状筋膜的神经支配区，对brevican的免疫染色增加了4天，在4周后达到最大值，并且在病变后长达6个月仍可检测到。电子显微镜免疫细胞化学表明，brevican是细胞外基质区室的组成部分。在病变部位，观察到类似的brevican上调的时间过程。这些数据表明，布雷维康在脑损伤区域和病变神经支配区域上调。他们提出了brevican在反应性神经胶质增生中的作用，并且与brevican参与失神经区域的突触重组有关的假说相符。

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