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首页> 外文期刊>The European Journal of Neuroscience >Bone morphogenetic protein-7 enhances dendritic growth and receptivity to innervation in cultured hippocampal neurons.
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Bone morphogenetic protein-7 enhances dendritic growth and receptivity to innervation in cultured hippocampal neurons.

机译：骨形态发生蛋白7增强了树突状细胞的生长，并增强了海马神经元的神经支配能力。

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Members of the bone morphogenetic protein (BMP) family of growth factors are present in the central nervous system during development and throughout life. They are known to play an important regulatory role in cell differentiation, but their function in postmitotic telencephalic neurons has not been investigated. To address this question, we examined cultured hippocampal neurons following treatment with bone morphogenetic protein-7 (BMP-7, also referred to as osteogenic protein-1). When added at the time of plating, BMP-7 markedly stimulated the rate of dendritic development. Within 1 day, the dendritic length of BMP-7-treated neurons was more than twice that of controls. By three days the dendritic arbors of BMP-7-treated neurons had attained a level of branching similar to that of 2-week-old neurons cultured under standard conditions. Several findings indicate that BMP-7 selectively enhances dendritic development. While dendritic length was significantly increased in BMP-7-treated neurons, the length of the axon was not. In addition, the mRNA encoding the dendritic protein MAP2 was significantly increased by BMP-7 treatment, but the mRNA for tubulin was not. Finally, BMP-7 did not enhance cell survival. Because dendritic maturation is a rate-limiting step in synapse formation in hippocampal cultures, we examined whether BMP-7 accelerated the rate at which neurons became receptive to innervation. Using two separate experimental paradigms, we found that the rate of synapse formation (assessed by counting synapsin I-positive presynaptic vesicle clusters) was increased significantly in neurons that had been exposed previously to BMP-7. Because BMP-7 and related BMPs are expressed in the hippocampus in situ, these factors may play a role in regulating dendritic branching and synapse formation in both development and plasticity.
机译：骨骼形态发生蛋白（BMP）生长因子家族的成员在发育过程中和一生中都存在于中枢神经系统中。已知它们在细胞分化中起重要的调节作用，但尚未研究其在有丝分裂后脑神经元中的功能。为了解决这个问题，我们在用骨形态发生蛋白7（BMP-7，也称为成骨​​蛋白1）治疗后检查了培养的海马神经元。当在电镀时添加时，BMP-7显着刺激了树突发展的速度。在1天之内，BMP-7处理的神经元的树突长度是对照的两倍以上。到三天时，BMP-7处理过的神经元的树突状树突达到了与标准条件下培养的2周龄神经元相似的分支水平。几个发现表明BMP-7选择性增强树突的发育。虽然BMP-7处理的神经元的树突长度明显增加，但轴突的长度却没有。此外，BMP-7处理可显着增加编码树突状蛋白MAP2的mRNA，而微管蛋白的mRNA则没有。最后，BMP-7不能提高细胞存活率。因为树突成熟是海马培养中突触形成的一个限速步骤，所以我们检查了BMP-7是否加速了神经元接受神经支配的速度。使用两个单独的实验范式，我们发现在先前接触过BMP-7的神经元中，突触形成的速率（通过计数突触素I阳性的突触前囊泡簇评估）显着增加。由于BMP-7和相关的BMP在海马中原位表达，因此这些因素可能在发育和可塑性中调节树突状分支和突触形成中发挥作用。

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