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首页> 外文期刊>The European Journal of Neuroscience >Stereological analysis of the reorganization of the dentate gyrus following entorhinal cortex lesion in mice.
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Stereological analysis of the reorganization of the dentate gyrus following entorhinal cortex lesion in mice.

机译：小鼠内嗅皮质损伤后齿状回重组的体视学分析。

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Abstract Denervation of the dentate gyrus by entorhinal cortex lesion has been widely used to study the reorganization of neuronal circuits following central nervous system lesion. Expansion of the non-denervated inner molecular layer (commissural/associational zone) of the dentate gyrus and increased acetylcholinesterase-positive fibre density in the denervated outer molecular layer have commonly been regarded as markers for sprouting following entorhinal cortex lesion. However, because this lesion extensively denervates the outer molecular layer and causes tissue shrinkage, stereological analysis is required for an accurate evaluation of sprouting. To this end we have performed unilateral entorhinal cortex lesions in adult C57BL/6J mice and have assessed atrophy and sprouting in the dentate gyrus using modern unbiased stereological techniques. Results revealed the expected increases in commissural/associational zone width and density of acetylcholinesterase-positive fibres on single brain sections. Yet, stereological analysis failed to demonstrate concomitant increases in layer volume or total acetylcholinesterase-positive fibre length. Interestingly, calretinin-positive fibres did grow beyond the border of the commissural/associational zone into the denervated layer and were regarded as sprouting axons. Thus, our data suggest that in C57BL/6J mice shrinkage of the hippocampus rather than growth of fibres underlies the two morphological phenomena most often cited as evidence of regenerative sprouting following entorhinal cortex lesion. Moreover, our data suggest that regenerative axonal sprouting in the mouse dentate gyrus following entorhinal cortex lesion may be best assessed at the single-fibre level.
机译：摘要内嗅皮质病变对齿状回的去神经作用已被广泛用于研究中枢神经系统病变后神经元回路的重组。齿状回的无神经支配的内分子层的扩展（接合/结合区）和无神经支配的外分子层中乙酰胆碱酯酶阳性纤维密度的增加通常被认为是内嗅皮质病变后发芽的标志。但是，由于该病变广泛地破坏了外部分子层的神经并导致组织收缩，因此需要进行立体分析以准确评估萌芽状态。为此，我们在成年C57BL / 6J小鼠中进行了单侧内脏皮质损伤，并使用现代无偏立体学技术评估了齿状回中的萎缩和发芽。结果显示，在单脑切片上，连合/结合区宽度和乙酰胆碱酯酶阳性纤维密度的预期增加。然而，立体分析未能证明层体积或总乙酰胆碱酯酶阳性纤维长度的同时增加。有趣的是，钙网蛋白阳性纤维的确生长超出了连合/结合区的边界，进入了神经支配层，并被视为发芽轴突。因此，我们的数据表明，在C57BL / 6J小鼠中，海马收缩而不是纤维生长是两种形态学现象的基础，而这两种现象最常被引用为内嗅皮质病变后再生发芽的证据。此外，我们的数据表明，内嗅皮质损伤后小鼠齿状回中的再生轴突发芽最好在单纤维水平上评估。

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