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首页> 外文期刊>The European Journal of Neuroscience >Functional maturation of isolated neural progenitor cells from the adult rat hippocampus.
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Functional maturation of isolated neural progenitor cells from the adult rat hippocampus.

机译：来自成年大鼠海马体的分离的神经祖细胞的功能成熟。

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Abstract Although neural progenitor cells (NPCs) may provide a source of new neurons to alleviate neural trauma, little is known about their electrical properties as they differentiate. We have previously shown that single NPCs from the adult rat hippocampus can be cloned in the presence of heparan sulphate chains purified from the hippocampus, and that these cells can be pushed into a proliferative phenotype with the mitogen FGF2 [Chipperfield, H., Bedi, K.S., Cool, S.M. & Nurcombe, V. (2002) Int. J. Dev. Biol., 46, 661-670]. In this study, the active and passive electrical properties of both undifferentiated and differentiated adult hippocampal NPCs, from 0 to 12 days in vitro as single-cell preparations, were investigated. Sparsely plated, undifferentiated NPCs had a resting membrane potential of approximately -90 mV and were electrically inexcitable. In > 70%, ATP and benzoylbenzoyl-ATP evoked an inward current and membrane depolarization, whereas acetylcholine, noradrenaline, glutamate and GABA had no detectable effect. In Fura-2-loaded undifferentiated NPCs, ATP and benzoylbenzoyl-ATP evoked a transient increase in the intracellular free Ca(2+) concentration, which was dependent on extracellular Ca(2+) and was inhibited reversibly by pyridoxalphosphate-6-azophenyl-2'-4'-disulphonic acid (PPADS), a P2 receptor antagonist. After differentiation, NPC-derived neurons became electrically excitable, expressing voltage-dependent TTX-sensitive Na(+) channels, low- and high-voltage-activated Ca(2+) channels and delayed-rectifier K(+) channels. Differentiated cells also possessed functional glutamate, GABA, glycine and purinergic (P2X) receptors. Appearance of voltage-dependent and ligand-gated ion channels appears to be an important early step in the differentiation of NPCs.
机译：摘要尽管神经祖细胞（NPC）可能为减轻神经损伤提供了新的神经元来源，但由于它们的分化，对其电学特性知之甚少。先前我们已经证明，成年大鼠海马中的单个NPC可以在从海马中纯化的硫酸乙酰肝素链存在下克隆，并且这些细胞可以与促分裂原FGF2一起推入增殖表型[Chipperfield，H.，Bedi， KS，酷，SM ＆Nurcombe，V.（2002），国际J.开发生物化学杂志，46，661-670]。在这项研究中，研究了未分化和分化的成年海马NPC作为单细胞制剂在体外0到12天的主动和被动电特性。稀疏电镀的未分化NPC的静息膜电位约为-90 mV，在电学上是不能激发的。在> 70％的浓度下，ATP和苯甲酰苯甲酰-ATP引起内向电流和膜去极化，而乙酰胆碱，去甲肾上腺素，谷氨酸和GABA则没有检测到作用。在富拉2加载未分化的NPC中，ATP和苯甲酰基苯甲酰基-ATP引起细胞内游离Ca（2+）浓度的瞬时增加，这取决于细胞外Ca（2+），并被吡ido醛磷酸盐-6-偶氮苯基-可逆地抑制。 2'-4'-二磺酸（PPADS），一种P2受体拮抗剂。分化后，NPC衍生的神经元变得电兴奋性，表达电压依赖性TTX敏感的Na（+）通道，低和高电压激活的Ca（2+）通道和延迟整流器K（+）通道。分化的细胞还具有功能性谷氨酸，GABA，甘氨酸和嘌呤能（P2X）受体。电压依赖性和配体门控离子通道的出现似乎是NPC分化的重要早期步骤。

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