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首页> 外文期刊>The European Journal of Neuroscience >Down's syndrome astrocytes have greater antioxidant capacity than euploid astrocytes.
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Down's syndrome astrocytes have greater antioxidant capacity than euploid astrocytes.

机译：唐氏综合症星形胶质细胞比整倍体星形胶质细胞具有更大的抗氧化能力。

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Down's syndrome (trisomy 21) brain tissue is considered to be susceptible to oxidative injury, mainly because its increased Cu/Zn-superoxide dismutase (SOD1) activity is not followed by an adaptive rise in hydrogen peroxide metabolizing enzymes. In vitro, trisomic neurons suffer oxidative stress and degenerate. We studied the response of trisomy 21 neuron and astrocyte cultures to hydrogen peroxide injury and found that they were, respectively, more and less vulnerable than their euploid counterparts. Differences were detected 24 h after exposures in the region of 50 microm and 500 microm hydrogen peroxide for neuron and astrocyte cultures, respectively. Cytotoxicity results were paralleled by a decrease in cellular glutathione. In addition, trisomic astrocytes showed a lower basal content of superoxide ion and a higher clearance of hydrogen peroxide from the culture medium. In the presence of hydrogen peroxide, trisomic astrocytes maintained their concentration of intracellular superoxide and hydroperoxides at a lower level than euploid astrocytes. Consistent with these results, trisomic astrocytes in neuron coculture were more neuroprotective than euploid astrocytes against hydrogen peroxide injury. We suggest that SOD1 overexpression has beneficial effects on astrocytes, as it does in other systems with similarly high disposal of hydroperoxides. In addition to a higher enzymatic activity of SOD1, cultures of trisomic astrocytes showed slightly higher glutathione reductase activity than euploid cultures. Thus, trisomy 21 astrocytes showed a greater antioxidant capacity against hydrogen peroxide than euploid astrocytes, and they partially counteracted the oxidative vulnerability of trisomic neurons in culture.
机译：唐氏综合症（21三体综合征）脑组织被认为易受氧化损伤，主要是因为其增加的Cu / Zn-超氧化物歧化酶（SOD1）活性并未随之过氧化氢代谢酶的适应性升高。在体外，三体神经元遭受氧化应激并退化。我们研究了21三体神经元和星形胶质细胞培养物对过氧化氢损伤的反应，发现它们分别比整倍体更易受伤害。在神经元和星形胶质细胞培养分别暴露于50微米和500微米的过氧化氢区域后24小时，检测到差异。细胞毒性结果与细胞内谷胱甘肽的减少平行。另外，三体星形胶质细胞显示出较低的基础超氧化物离子含量和较高的过氧化氢从培养基中的清除率。在过氧化氢的存在下，三体星形胶质细胞保持其细胞内超氧化物和氢过氧化物的浓度低于整倍体星形胶质细胞。与这些结果一致，神经元共培养中的三体星形胶质细胞比整倍体星形胶质细胞对过氧化氢的损伤更具神经保护作用。我们建议，SOD1过表达对星形胶质细胞具有有益的作用，就像在其他具有过氧化氢过高处理能力的系统中一样。除了具有较高的SOD1酶活性外，三体星形胶质细胞的培养物中谷胱甘肽还原酶的活性也比整倍体培养物高。因此，三体性21星形胶质细胞比整倍体星形胶质细胞具有更大的抗过氧化氢的抗氧化能力，并且它们部分抵消了培养物中三体神经元的氧化脆弱性。

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