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首页> 外文期刊>The European Journal of Neuroscience >Differential distribution of calpain small subunit 1 and 2 in rat brain.
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Differential distribution of calpain small subunit 1 and 2 in rat brain.

机译：钙蛋白酶小亚基1和2在大鼠脑中的差异分布。

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摘要

Abstract Calpains, the Ca(2+)-dependent thiol proteases, are abundant in the nervous tissue. The ubiquitous enzyme forms in mammals are heterodimers consisting of a specific, micro or m, large (catalytic) subunit and, apparently, a common small (regulatory) subunit (CSS1). Recently, however, we described a second form of small subunit (CSS2), which is of restricted occurrence [Schad, E., Farkas, A., Jekely, G., Tompa, P. & Friedrich, P. (2002) Biochem. J., 362, 383-388]. Here we analysed the distribution of immunoreactivity in various parts of rat brain against two anti-CSS1 and two anti-CSS2 antibodies by correlated light and electron microscopy. Remarkably, the antibodies showed differential distribution in various parts of rat cortex: anti-CSS1 reacted mainly with perikarya and dendrites, whereas anti-CSS2 was more prominent in axons. In serial sections CSS2 and synaptophysin gave very similar patterns, i.e. these epitopes seem to colocalize. Electron microscopy confirmed that CSS1 was mainly localized postsynaptically in dendrites and somata, whereas CSS2 was found presynaptically. The hypothesis is advanced that these distinct distributions of calpain subunits may be related to the transport of these enzymes in nerve cells.
机译：摘要钙蛋白酶是一种依赖Ca（2+）的硫醇蛋白酶，在神经组织中含量很高。哺乳动物中普遍存在的酶形式是异源二聚体，其由一个特定的，微小的或微米的大（催化）亚基，以及一个常见的小（调节）亚基（CSS1）组成。但是，最近，我们描述了第二种形式的小亚基（CSS2），这种第二种形式的发生受到限制[Schad，E.，Farkas，A.，Jekely，G.，Tompa，P.＆Friedrich，P.（2002）Biochem 。 J.，362，383-388]。在这里，我们通过相关的光学和电子显微镜分析了大鼠脑中针对两种抗CSS1和两种抗CSS2抗体的免疫反应性分布。值得注意的是，这些抗体在大鼠皮层的各个部位均表现出不同的分布：抗CSS1主要与皮核和树突反应，而抗CSS2在轴突中更为突出。在系列文章中，CSS2和突触素的模式非常相似，即这些表位似乎共定位。电子显微镜证实，CSS1主要在突触后位于树突和躯体中，而CSS2在突触前被发现。提出的假设是，钙蛋白酶亚基的这些不同分布可能与这些酶在神经细胞中的运输有关。

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