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首页> 外文期刊>The European Journal of Neuroscience >Distribution and regulation of L-type calcium channels in deep dorsal horn neurons after sciatic nerve injury in rats.
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Distribution and regulation of L-type calcium channels in deep dorsal horn neurons after sciatic nerve injury in rats.

机译：大鼠坐骨神经损伤后深背角神经元中L型钙通道的分布和调控。

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摘要

Deep dorsal horn neurons are involved in the processing of nociceptive information in the spinal cord. Previous studies revealed a role of the intrinsic bioelectrical properties (plateau potentials) of deep dorsal horn neuron in neuronal hyperexcitability, indicating their function in pain sensitization. These properties were considered to rely on L-type calcium currents. Two different isotypes of L-type calcium channel alpha 1 subunit have been cloned (Ca(V)1.2 and Ca(V)1.3). Both are known to be expressed in the spinal cord. However, no data were available on their subcellular localization. Moreover, possible changes in Ca(V)1.2 and Ca(V)1.3 expression had never been investigated in nerve injury models. Our study provides evidence for a differential expression of Ca(V)1.2 and Ca(V)1.3 subunits in the somato-dendritic compartment of deep dorsal horn neurons. Ca(V)1.2 immunoreactivity is restricted to the soma and proximal dendrites whereas Ca(V)1.3 immunoreactivity is found in the whole somato-dendritic compartment, up to distal dendritic segments. Moreover, these specific immunoreactive patterns are also found in electrophysiologically identified deep dorsal horn neurons expressing plateau potentials. After nerve injury, namely total axotomy or partial nerve ligation, Ca(V)1.2 and Ca(V)1.3 expression undergo differential changes, showing up- and down-regulation, respectively, both at the protein and at the mRNA levels. Taken together, our data support the role of L-type calcium channels in the control of intrinsic biolectrical regenerative properties. Furthermore, Ca(V)1.2 and Ca(V)1.3 subunits may have distinct and specific roles in sensory processing in the dorsal horn of the spinal cord, the former being most likely involved in long-term changes after nerve injury.
机译：深背角神经元参与脊髓伤害感受信息的处理。先前的研究揭示了深背角神经元的内在生物电特性（高原电位）在神经元过度兴奋中的作用，表明它们在疼痛致敏中的功能。这些性质被认为依赖于L型钙电流。 L型钙通道alpha 1亚基的两个不同的同种型已被克隆（Ca（V）1.2和Ca（V）1.3）。已知两者均在脊髓中表达。但是，没有关于其亚细胞定位的数据。而且，从未在神经损伤模型中研究过Ca（V）1.2和Ca（V）1.3表达的可能变化。我们的研究提供了深背角神经元的体树突状区室中Ca（V）1.2和Ca（V）1.3亚基差异表达的证据。 Ca（V）1.2免疫反应性仅限于躯体和近端树突，而Ca（V）1.3免疫反应性则存在于整个躯体树突状区室中，直至远端树突状节段。而且，在电生理学上鉴定的表达高原电位的深背角神经元中也发现了这些特定的免疫反应模式。神经损伤后，即全轴切或部分神经结扎，Ca（V）1.2和Ca（V）1.3的表达发生差异变化，分别在蛋白质和mRNA水平上表达上调和下调。两者合计，我们的数据支持L型钙通道在控制内在的生物电再生特性中的作用。此外，Ca（V）1.2和Ca（V）1.3亚基可能在脊髓背角的感觉处理中具有独特的特定作用，前者最有可能参与神经损伤后的长期变化。

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