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首页> 外文期刊>The European Journal of Neuroscience >Differential expression of KCC2 accounts for the differential GABA responses between relay and intrinsic neurons in the early postnatal rat olfactory bulb.
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Differential expression of KCC2 accounts for the differential GABA responses between relay and intrinsic neurons in the early postnatal rat olfactory bulb.

机译：KCC2的差异表达解释了早期产后大鼠嗅球中中继神经元和内在神经元之间的GABA反应差异。

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Abstract The rat olfactory bulb is anatomically immature at birth, and considerable neurogenesis and synaptogenesis are known to take place postnatally. In addition, significant physiological changes have also been reported in this period. For example, granule cell-mediated inhibition following electrical stimulations to the lateral olfactory tract is robust during the first postnatal week, and then decreases abruptly after the second week. However, the mechanism underlying this enhanced inhibition remains to be elucidated. To know the cause of this phenomenon, we investigated the expression patterns of cation-Cl(-) co-transporters (KCC1, KCC2 and NKCC1) mRNAs, which are responsible for the regulation of [Cl(-)](i). In addition, responses to gamma-aminobutyric acid (GABA) were measured by gramicidin-perforated patch-clamp recordings and Ca(2+) imaging using fura-2. We found that in the early postnatal period, mitral cells expressing KCC2 mRNA were inhibited by GABA, while granule cells lacking KCC2 mRNA expression were depolarized or excited by GABA. These results indicate that transient GABA-mediated excitation on granule cells might be the main cause of the enhanced inhibition on mitral cells, and suggest that these differential GABA responses between relay and intrinsic neurons play pivotal roles in the early postnatal rat olfactory bulb.
机译：摘要大鼠嗅球在出生时在解剖上是不成熟的，并且已知在出生后会发生大量的神经发生和突触发生。此外，在此期间也已报告了明显的生理变化。例如，在产后的第一个星期内，对嗅觉刺激的颗粒细胞介导的抑制作用很强，然后在第二个星期后突然下降。但是，这种抑制作用增强的机理尚待阐明。要知道这种现象的原因，我们研究了阳离子-Cl（-）共同转运蛋白（KCC1，KCC2和NKCC1）mRNA的表达模式，它们负责[Cl（-）]（i）的调控。此外，通过使用短链霉菌素穿孔的膜片钳记录和使用fura-2的Ca（2+）成像来测量对γ-氨基丁酸（GABA）的响应。我们发现，在出生后早期，表达KCC2 mRNA的二尖瓣细胞被GABA抑制，而缺乏KCC2 mRNA表达的颗粒细胞被GABA消极化或激发。这些结果表明，短暂的GABA介导的对颗粒细胞的兴奋可能是对二尖瓣细胞抑制作用增强的主要原因，并表明中继和内在神经元之间的这些差异性GABA反应在出生后大鼠早期嗅球中起关键作用。

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