...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Differential expression of calcineurin A subunit mRNA isoforms during rat hippocampal and cerebellar development.
【24h】

Differential expression of calcineurin A subunit mRNA isoforms during rat hippocampal and cerebellar development.

机译：钙调神经磷酸酶A亚基mRNA亚型在大鼠海马和小脑发育过程中的差异表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Calcineurin (protein phosphatase 2B) is a calcium-dependent serine-threonine phosphatase. It has diverse roles and is centrally involved in synaptic plasticity. The catalytic A subunit of calcineurin has three isoforms, alpha, beta and gamma. Their expression and ontogeny in the brain has not been systematically investigated; such data become important with a report that PPP3CC, the gene encoding calcineurin Agamma, is a susceptibility gene for schizophrenia, and the finding that its expression is decreased in the disorder. We used in situ hybridization histochemistry to measure the relative transcript abundance of calcineurin Agamma and the other catalytic isoforms, Aalpha and Abeta, during development of the Sprague-Dawley rat hippocampus and cerebellum. All three isoforms are present in both regions at all time points [embryonic day 19 (E19) to postnatal day 42 (P42)] and undergo developmental regulation, but differ in their ontogenic profile. Calcineurin Aalpha and Abeta mRNAs increased from E19 through to adulthood, whereas Agamma mRNA was most highly expressed during early developmental stages. Calcineurin Aalpha and Abeta mRNAs positively correlated with synaptophysin mRNA (a synaptic marker), whilst Agamma mRNA was either unrelated to, or negatively correlated, with this transcript. These data confirm that all three calcineurin A subunits are expressed in the rodent brain, and indicate that calcineurin Agamma may have different roles than Aalpha and Abeta. The data also suggest a potential importance of calcineurin Agamma in neurodevelopment, and in the genetically influenced neurodevelopmental disturbance that is thought to underlie schizophrenia.
机译：钙调神经磷酸酶（蛋白磷酸酶2B）是一种钙依赖性丝氨酸-苏氨酸磷酸酶。它具有不同的作用，并主要参与突触可塑性。钙调神经磷酸酶的催化​​A亚基具有三种同工型，α，β和γ。它们在大脑中的表达和个体发育尚未得到系统的研究。有报道称，编码钙调神经磷酸酶γ的PPP3CC基因是精神分裂症的易感基因，并且发现其表达在疾病中降低，因此这些数据变得重要。我们在Sprague-Dawley大鼠海马和小脑发育过程中，使用原位杂交组织化学方法测量了钙调神经磷酸酶Agamma和其他催化亚型Aalpha和Abeta的相对转录丰度。所有三个同工型都在所有时间点[胚胎第19天（E19）至出生后第42天（P42）]都存在于两个区域中，并经历发育调节，但它们的个体发生谱不同。从E19到成年，钙调磷酸酶Aalpha和Abeta mRNAs增加，而在发育初期，Agamma mRNA表达最高。钙调磷酸酶Aalpha和Abeta mRNA与突触素mRNA（一种突触标记）正相关，而Agamma mRNA与该转录本无关或负相关。这些数据证实了所有三个钙调神经磷酸酶A亚基均在啮齿动物的大脑中表达，并表明钙调神经磷酸酶Agamma可能具有与Aalpha和Abeta不同的作用。数据还表明，钙调神经磷酸酶γ在神经发育中以及在认为是精神分裂症的遗传影响的神经发育障碍中具有潜在的重要性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号