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首页> 外文期刊>The European Journal of Neuroscience >Differences between cation-chloride co-transporter functions in the visual cortex of pigmented and albino rats.
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Differences between cation-chloride co-transporter functions in the visual cortex of pigmented and albino rats.

机译：色素和白化病大鼠视皮层中阳离子-氯化物共转运蛋白功能之间的差异。

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Abstract Albinism in mammals is accompanied by specific morphological and functional alterations of the visual system. To understand their cellular basis we studied the physiological characteristics and transmembrane currents of pyramidal neurons in 350-microm-thick slices of visual cortex from pigmented and albino rats using whole-cell and gramicidin perforated patch-clamp recordings. The resting membrane potential was significantly more positive and the rheobase was significantly lower in neurons of layers II/III and V in albinos as compared with pigmented rats. No significant differences were found in the input resistance, time constant and chronaxy. Whereas the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor-mediated currents were not significantly different, the maximum gamma-aminobutyric acid (GABA)(A) receptor (GABA(A)R)-mediated currents and miniature inhibitory postsynaptic currents showed significantly lower amplitudes in neurons of layer V in visual cortex of albinos as compared with pigmented rats. The reversal potential of the GABA(A)R-mediated currents (E(GABA)) was significantly shifted to more positive values in albinos. Pharmacological experiments showed that this shift could be caused by an increased action of the inward chloride co-transporter NKCC1 and reduced action of the outward chloride co-transporter KCC2 in albino rats. This difference seems to be restricted to the visual cortex because in pyramidal neurons from frontal cortex E(GABA) was not significantly different in albinos as compared with pigmented rats. These results are discussed in relation to functional alterations in the albino visual system.
机译：摘要哺乳动物中的白化病伴有视觉系统的特定形态和功能改变。为了了解它们的细胞基础，我们使用全细胞和青霉菌素穿孔的膜片钳记录，研究了有色和白化病大鼠的350微米厚的视觉皮层中锥体神经元的生理特征和跨膜电流。与有色大鼠相比，白化病患者II / III和V层神经元的静息膜电位明显更高，而流变碱则明显更低。在输入电阻，时间常数和时差方面没有发现显着差异。尽管α-氨基-3-羟基-5-甲基-4-异恶唑丙酸受体介导的电流没有显着差异，但最大的γ-氨基丁酸（GABA）（A）受体（GABA（A）R）介导的电流和有色抑制的突触后电流显示，与有色大鼠相比，白化病患者视觉皮层V层神经元的振幅明显降低。在白化病患者中，GABA（A）R介导的电流（E（GABA））的逆转电位已显着转变为更多的正值。药理实验表明，这种转变可能是由于白化病大鼠内向氯化物共转运蛋白NKCC1的作用增强和外向氯化物共转运蛋白KCC2的作用减少所致。这种差异似乎仅限于视觉皮层，因为额叶皮层的锥体神经元E（GABA）在白化病患者中与有色大鼠相比没有显着差异。讨论了这些结果与白化病视觉系统中的功能变化有关。

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