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首页> 外文期刊>The European Journal of Neuroscience >Exercise affects energy metabolism and neural plasticity-related proteins in the hippocampus as revealed by proteomic analysis.
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Exercise affects energy metabolism and neural plasticity-related proteins in the hippocampus as revealed by proteomic analysis.

机译：蛋白质组学分析表明，运动会影响海马体的能量代谢和神经可塑性相关蛋白。

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摘要

Studies were conducted to evaluate the effect of a brief voluntary exercise period on the expression pattern and post-translational modification of multiple protein classes in the rat hippocampus using proteomics. An analysis of 80 protein spots of relative high abundance on two-dimensional gels revealed that approximately 90% of the proteins identified were associated with energy metabolism and synaptic plasticity. Exercise up-regulated proteins involved in four aspects of energy metabolism, i.e. glycolysis, ATP synthesis, ATP transduction and glutamate turnover. Specifically, we found increases in fructose-bisphosphate aldolase C, phosphoglycerate kinase 1, mitochondrial ATP synthase, ubiquitous mitochondrial creatine kinase and glutamate dehydrogenase 1. Exercise also up-regulated specific synaptic-plasticity-related proteins, the cytoskeletal protein alpha-internexin and molecular chaperones (chaperonin-containing TCP-1, neuronal protein 22, heat shock 60-kDa protein 1 and heat shock protein 8). Western blot was used to confirm the direction and magnitude of change in ubiquitous mitochondrial creatine kinase, an enzyme essential for transducing mitochondrial-derived ATP to sites of high-energy demand such as the synapse. Protein phosphorylation visualized by Pro-Q Diamond fluorescent staining showed that neurofilament light polypeptide, glial fibrillary acidic protein, heat shock protein 8 and transcriptional activator protein pur-alpha were more intensely phosphorylated with exercise as compared with sedentary control levels. Our results, together with the fact that most of the proteins that we found to be up-regulated have been implicated in cognitive function, support a mechanism by which exercise uses processes of energy metabolism and synaptic plasticity to promote brain health.
机译：进行了研究，以评估短暂的自愿运动期对使用蛋白质组学的大鼠海马中多种蛋白质类别的表达模式和翻译后修饰的影响。在二维凝胶上对80个相对较高丰度的蛋白质斑点进行分析后发现，鉴定出的蛋白质中大约90％与能量代谢和突触可塑性有关。锻炼上调的蛋白质，参与能量代谢的四个方面，即糖酵解，ATP合成，ATP转导和谷氨酸转换。具体而言，我们发现果糖二磷酸醛缩酶C，磷酸甘油酸激酶1，线粒体ATP合酶，普遍存在的线粒体肌酸激酶和谷氨酸脱氢酶1增加。运动还上调了特定的突触可塑性相关蛋白，细胞骨架蛋白α-内毒素和分子。分子伴侣（含伴侣蛋白的TCP-1，神经元蛋白22，热休克60-kDa蛋白1和热休克蛋白8）。 Western印迹法用于确定普遍存在的线粒体肌酸激酶的变化方向和幅度，该酶是将线粒体来源的ATP转导至高能量需求位点（如突触）所必需的。通过Pro-Q Diamond荧光染色可视化的蛋白质磷酸化显示，与久坐的对照水平相比，运动时神经丝轻多肽，神经胶质原纤维酸性蛋白，热休克蛋白8和转录激活蛋白pur-alpha的磷酸化程度更高。我们的研究结果以及我们发现被上调的大多数蛋白质与认知功能有关的事实，支持了一种机制，通过该机制运动可以利用能量代谢过程和突触可塑性促进大脑健康。

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