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首页> 外文期刊>The European Journal of Neuroscience >Distinct expression of Cbln family mRNAs in developing and adult mouse brains.
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Distinct expression of Cbln family mRNAs in developing and adult mouse brains.

机译：Cbln家族mRNA在发育中和成年小鼠大脑中的不同表达。

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Cbln1 belongs to the C1q and tumour necrosis factor superfamily, and plays crucial roles as a cerebellar granule cell-derived transneuronal regulator for synapse integrity and plasticity in Purkinje cells. Although Cbln2-Cbln4 are also expressed in the brain and could form heteromeric complexes with Cbln1, their precise expressions remain unclear. Here, we investigated gene expression of the Cbln family in developing and adult C57BL mouse brains by reverse transcriptase-polymerase chain reaction (RT-PCR), Northern blot, and high-resolution in situ hybridization (ISH) analyses. In the adult brain, spatial patterns of mRNA expression were highly differential depending on Cbln subtypes. Notably, particularly high levels of Cbln mRNAs were expressed in some nuclei and neurons, whereas their postsynaptic targets often lacked or were low for any Cbln mRNAs, as seen for cerebellar granule cells/Purkinje cells, entorhinal cortex/hippocampus, intralaminar group of thalamic nuclei/caudate-putamen, and dorsal nucleus of the lateral lemniscus/central nucleus of the inferior colliculus. In the developing brain, Cbln1, 2, and 4 mRNAs appeared as early as embryonic day 10-13, and exhibited transient up-regulation during the late embryonic and neonatal periods. For example, Cbln2 mRNA was expressed in the cortical plate of the developing neocortex, displaying a high rostromedial to low caudolateral gradient. In contrast, Cbln3 mRNA was selective to cerebellar granule cells throughout development, and its onset was as late as postnatal day 7-10. These results will provide a molecular-anatomical basis for future studies that characterize roles played by the Cbln family.
机译：Cbln1属于C1q和肿瘤坏死因子超家族，并作为小脑颗粒细胞源性跨神经调节剂，在浦肯野细胞中突触的完整性和可塑性中起着至关重要的作用。尽管Cbln2-Cbln4也可以在大脑中表达，并可能与Cbln1形成异源复合物，但它们的确切表达仍不清楚。在这里，我们通过逆转录聚合酶链反应（RT-PCR），Northern印迹和高分辨率原位杂交（ISH）分析了发育中和成年C57BL小鼠大脑中Cbln家族的基因表达。在成年大脑中，取决于Cbln亚型，mRNA表达的空间模式差异很大。值得注意的是，在某些核和神经元中，Cbln mRNA的表达特别高，而对于任何Cbln mRNA，它们的突触后靶点通常缺乏或低，如小脑颗粒细胞/浦肯野细胞，内嗅皮层/海马，丘脑核的层内组所见/尾状丘脑，以及外侧弯月盘的背核/下丘的中央核。在发育中的大脑中，Cbln1、2和4 mRNA早在胚胎的第10-13天就出现了，并且在胚胎和新生儿后期表现出短暂的上调。例如，Cbln2 mRNA在发育中的新皮层的皮质板中表达，表现出从高rostromedial到低caudolateral梯度。相比之下，Cbln3 mRNA在整个发育过程中对小脑颗粒细胞具有选择性，其发作时间晚至出生后7-10天。这些结果将为未来研究表征Cbln家族所扮演的角色提供分子解剖学基础。

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