...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >P2Y1 and P2X7 receptors induce calcium/calmodulin-dependent protein kinase II phosphorylation in cerebellar granule neurons.
【24h】

P2Y1 and P2X7 receptors induce calcium/calmodulin-dependent protein kinase II phosphorylation in cerebellar granule neurons.

机译：P2Y1和P2X7受体在小脑颗粒神经元中诱导钙/钙调蛋白依赖性蛋白激酶II磷酸化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The activation of nucleotide receptors-- both ionotropic, P2X, and most of metabotropic, P2Y-- increases intracellular calcium concentration, resulting in calcium/calmodulin-dependent protein kinase II (CaMKII) activation. Stimulation of cerebellar granule neurons in culture-- with different P2X and P2Y agonists and their effect on CaMKII phosphorylation-- was studied using immunocytochemical and microfluorimetrical techniques. P2X agonist: 2'-3'-o-(4-benzoylbenzoyl)-adenosine 5'-triphosphate (BzATP), alpha,beta-methylene adenosine 5'-triphosphate (alpha,beta-meATP) and diadenosine pentaphosphate (Ap(5)A); and P2Y agonists: 2-(methylthyo)-adenosine diphosphate (2MeSADP) and uridine 5'-bisphosphate (UDP); tested induced a CaMKII phosphorylation but with a different immunostaining pattern in each group. Stimulation with 2MeSADP induced a Ca(2+) release from intracellular stores and a significant CaMKII phosphorylation in cell somas and neurites. This agrees with the subcellular distribution of P2Y(1). MRS 2179, a specific P2Y(1) inhibitor, antagonized the 2MeSADP effect. On the other hand, cerebellar granule neuron stimulation with BzATP, in Mg(2+)-free conditions, produced extracellular calcium entrance and, as a result, a significant increase in CaMKII phosphorylation mostly in fibres, which correspond with P2X(7) subdistribution. Immunocytochemical and microfluorimetrical experiments, using Zn(2+) and Brilliant Blue G (BBG), as a specific P2X(7) antagonist, confirmed that BzATP was acting through the P2X(7) receptor. These results indicate that P2Y(1) and P2X(7) produce a significant increase in CaMKII phosphorylation, but show important differences in subcellular distribution and in effect duration. P2X(7) activation in granule neurons is not associated with pore formation, according to the absence of YO-PRO-1 fluorescence. The abundant presence of P2X(7) at the synaptic structures suggests a relevant role played by this receptor in synaptic plasticity.
机译：离子受体P2X和大多数代谢型P2Y核苷酸受体的激活会增加细胞内钙的浓度，从而导致钙/钙调蛋白依赖性蛋白激酶II（CaMKII）的激活。使用免疫细胞化学和微荧光技术研究了使用不同的P2X和P2Y激动剂刺激培养物中的小脑颗粒神经元及其对CaMKII磷酸化的影响。 P2X激动剂：2'-3'-邻-（4-苯甲酰基苯甲酰基）-腺苷5'-三磷酸（BzATP），α，β-亚甲基腺苷5'-三磷酸（alpha，β-meATP）和二磷酸腺苷五磷酸（Ap（5 ）一种）;和P2Y激动剂：2-（甲基硫代）-腺苷二磷酸（2MeSADP）和尿苷5'-二磷酸（UDP）;测试组诱导CaMKII磷酸化，但每组的免疫染色模式不同。用2MeSADP刺激诱导Ca（2+）释放从细胞内的存储区和明显的CaMKII磷酸化在细胞体和神经突中。这与P2Y（1）的亚细胞分布一致。 MRS 2179，一种特定的P2Y（1）抑制剂，拮抗2MeSADP的作用。另一方面，在无Mg（2+）的条件下，用BzATP刺激小脑颗粒神经元会产生细胞外钙进入，因此，CaMKII磷酸化的显着增加主要是在纤维中，这与P2X（7）的亚分布相对应。免疫细胞化学和微荧光实验，使用Zn（2+）和亮蓝G（BBG）作为特定的P2X（7）拮抗剂，证实BzATP通过P2X（7）受体起作用。这些结果表明P2Y（1）和P2X（7）产生CaMKII磷酸化的显着增加，但在亚细胞分布和作用时间上显示出重要差异。根据缺少YO-PRO-1荧光，颗粒神经元中的P2X（7）激活与孔形成无关。 P2X（7）在突触结构的大量存在表明该受体在突触可塑性中发挥了相关作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号