...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Distribution of TRPC1 receptors in dendrites of rat substantia nigra: a confocal and electron microscopy study.
【24h】

Distribution of TRPC1 receptors in dendrites of rat substantia nigra: a confocal and electron microscopy study.

机译：大鼠黑质树突状体中TRPC1受体的分布：共聚焦和电子显微镜研究。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Transient receptor potential channels (TRPC) are plasma membrane, non-selective cationic channels and have been proposed as candidates involved in the regulation of cellular Ca(2+) influx. The expression, at mRNA level, of several TRPCs has been demonstrated recently in dopaminergic neurons of the substantia nigra (SN). The aim of the present study was to characterize the expression of TRPC1, at a protein level, in the substantia nigra neurons and non-excitable cells of Wistar rats. Single-label immunohistochemistry and double-label immunofluorescence were used to study the expression of TRPC1 among substantia nigra dopamine neurons and cellular processes using antibodies against tyrosine hydroxylase (TH), substance P (SP), enkephalin, synaptophysin, vesicular glutamate transporter-2 (Vglut-2), microtubule associated protein-2 and metabotropic glutamate receptor 1 (mGluR1). Moreover, the ultrastructural localization of TRPC1 was investigated by means of electron microscopy. A set of dual label experiments was also performed to investigate the presence of TRPC1 among glial cells. Our results showed that TRPC1 is localized mainly in dendritic processes of dopamine neurons, whereas a relatively small percentage of neuronal somata display a light TRPC1 immunoreactivity. Such results were confirmed by our electron microscopy observations. Our study demonstrates, for the first time, a coexpression of TRPC1 and mGluR1 receptors in dendrites of the substantia nigra dopaminergic neurons. Such observation reinforces the concept of an involvement of TRPC1 in mGluR1-mediated excitatory inputs in rat dopamine neurons.
机译：瞬态受体电位通道（TRPC）是质膜，非选择性阳离子通道，并已被提议作为参与细胞Ca（2+）内流调节的候选者。最近在黑质（SN）的多巴胺能神经元中证实了几种TRPC的mRNA水平表达。本研究的目的是在蛋白水平上表征Wistar大鼠黑质神经元细胞和非兴奋性细胞中TRPC1的表达。使用抗酪氨酸羟化酶（TH），P物质（SP），脑啡肽，突触素，囊泡谷氨酸转运蛋白2（抗体），使用单标记免疫组化和双标记免疫荧光研究黑质多巴胺神经元和细胞过程中TRPC1的表达。 Vglut-2），微管相关蛋白2和代谢型谷氨酸受体1（mGluR1）。此外，通过电子显微镜研究了TRPC1的超微结构定位。还进行了一组双标记实验，以研究神经胶质细胞中TRPC1的存在。我们的结果表明，TRPC1主要定位在多巴胺神经元的树突状过程中，而相对较少百分比的神经元体细胞显示出轻的TRPC1免疫反应性。我们的电子显微镜观察证实了这样的结果。我们的研究首次证明了黑质多巴胺能神经元树突中的TRPC1和mGluR1受体共表达。这种观察加强了TRPC1参与大鼠多巴胺神经元中mGluR1介导的兴奋性输入的概念。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号