...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Pro-opiomelanocortin colocalizes with corticotropin- releasing factor in axon terminals of the noradrenergic nucleus locus coeruleus.
【24h】

Pro-opiomelanocortin colocalizes with corticotropin- releasing factor in axon terminals of the noradrenergic nucleus locus coeruleus.

机译：促黑素皮质激素原与促肾上腺皮质激素释放因子在去甲肾上腺素能核蓝斑轴突末端共定位。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We previously demonstrated that the opioid peptide enkephalin and corticotropin-releasing factor (CRF) are occasionally colocalized in individual axon terminals but more frequently converge on common dendrites in the locus coeruleus (LC). To further examine potential opioid cotransmitters in CRF afferents we investigated the distribution of pro-opiomelanocortin (POMC), the precursor that yields the potent bioactive peptide beta-endorphin, with respect to CRF immunoreactivity using immunofluorescence and immunoelectron microscopic analyses of the LC. Coronal sections were collected through the dorsal pontine tegmentum of rat brain and processed for immunocytochemical detection of POMC and CRF or tyrosine hydroxylase (TH). POMC-immunoreactive processes exhibited a distinct distribution within the LC as compared to the enkephalin family of opioid peptides. Specifically, POMC fibers were enriched in the ventromedial aspect of the LC with fewer fibers present dorsolaterally. Immunofluorescence microscopy showed frequent coexistence of POMC and CRF in varicose processes that overlapped TH-containing somatodendritic processes in the LC. Ultrastructural analysis showed POMC immunoreactivity in unmyelinated axons and axon terminals. Axon terminals containing POMC were filled with numerous large dense-core vesicles. In sections processed for POMC and TH, approximately 29% of POMC-containing axon terminals (n = 405) targeted dendrites that exhibited immunogold-silver labeling for TH. In contrast, sections processed for POMC and CRF showed that 27% of POMC-labeled axon terminals (n = 657) also exhibited CRF immunoreactivity. Taken together, these data indicate that a subset of CRF afferents targeting the LC contain POMC and may be positioned to dually impact LC activity.
机译：我们以前证明了阿片肽脑啡肽和促肾上腺皮质激素释放因子（CRF）有时在单个轴突末端共定位，但更频繁地会聚在蓝斑斑（LC）的常见树突中。为了进一步检查CRF传入细胞中潜在的阿片类辅助递质，我们使用LC的免疫荧光和免疫电子显微镜分析了C-细胞免疫反应性，研究了产生有效生物活性肽β-内啡肽的前阿片黑皮质素（POMC）的分布。通过大鼠脑背桥盖膜收集冠状切片，并进行免疫细胞化学检测POMC和CRF或酪氨酸羟化酶（TH）。与脑啡肽类阿片肽相比，POMC免疫反应过程在LC中表现出明显的分布。具体而言，POMC纤维在LC的腹侧富集，而背外侧的纤维较少。免疫荧光显微镜检查显示，在曲张过程中，POMC和CRF经常共存，与LC中的含TH的树突状过程重叠。超微结构分析显示未髓鞘的轴突和轴突末端的POMC免疫反应性。含有POMC的轴突末端充满了许多大的密芯囊泡。在为POMC和TH处理的切片中，大约29％的含POMC的轴突末端（n = 405）靶向树突，这些树突显示出TH的免疫金银标记。相反，对POMC和CRF进行处理的切片显示，有27％的POMC标记的轴突末端（n = 657）也具有CRF免疫反应性。综上所述，这些数据表明靶向LC的CRF传入子集包含POMC，并且可能被定位为双重影响LC活动。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号