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首页> 外文期刊>The European Journal of Neuroscience >Developmental changes of neurotrophin mRNA expression in the layers of rat visual cortex.
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Developmental changes of neurotrophin mRNA expression in the layers of rat visual cortex.

机译：大鼠视皮层各层神经营养蛋白mRNA表达的发育变化。

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摘要

Neurotrophins are essential factors for the structural, neurochemical and functional maturation of the brain including developmental and adult plasticity. Northern blots and polymerase chain reaction revealed the expression of neurotrophin 4 (NT4), neurotrophin 3 (NT3), brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in the cortex. The cellular producers of NT3 and BDNF have been characterized by anatomical methods as being mostly pyramidal, and the tyrosine kinase B (TrkB) receptor is expressed by many cortical neurons. However, these methods have so far failed to identify the cells producing NT4 and NGF mRNA. These factors are much lower in expression than, e.g. BDNF, and apparently remain below detection levels of in situ hybridization. Given their specific actions on cell types and afferent systems, knowledge about the producing cell types is highly desirable. To narrow down on the producing cell types, we quantified by reverse transcriptase-polymerase chain reaction (RT-PCR)the developmental changes of BDNF, NT3, NT4, NGF and TrkB mRNA expression in total visual cortex lysates, and in the cortical layers dissected by tangential cryostat sectioning. We found dramatic changes in laminar expression of NT3 and NGF, mild changes of NT4, and no changes of BDNF and TrkB mRNA. For instance, NT3 is important early on for thalamocortical axons, and we found transient peaks of NT3 mRNA expression first in layer VI, then in layer IV. NT4 mRNA was in layers IV and VI, suggesting NT4 protein production in thalamorecipient layers, but peak expression gradually shifted to upper layers as did NGF expression. The layer-specific developmental expression shifts of neurotrophin mRNAs correlate with morphogenetic processes.
机译：神经营养蛋白是大脑结构，神经化学和功能成熟（包括发育和成人可塑性）的重要因素。 Northern印迹和聚合酶链反应揭示了皮质中神经营养蛋白4（NT4），神经营养蛋白3（NT3），脑源性神经营养因子（BDNF）和神经生长因子（NGF）的表达。 NT3和BDNF的细胞产生者已通过解剖学方法鉴定为主要呈锥体状，酪氨酸激酶B（TrkB）受体由许多皮质神经元表达。然而，迄今为止，这些方法未能鉴定出产生NT4和NGF mRNA的细胞。这些因子在表达上比例如BDNF，并且显然保持在原位杂交的检测水平以下。考虑到它们对细胞类型和传入系统的特定作用，非常需要有关产生细胞类型的知识。为了缩小生产细胞的类型，我们通过逆转录酶-聚合酶链反应（RT-PCR）量化了总视觉皮层裂解液和解剖的皮层中BDNF，NT3，NT4，NGF和TrkB mRNA表达的发育变化。通过切线低温恒温器切片。我们发现NT3和NGF的层状表达，NT4的轻度变化以及BDNF和TrkB mRNA的变化均无明显变化。例如，NT3在早期对于丘脑皮层轴突很重要，我们首先在VI层，然后在IV层中发现NT3 mRNA表达的瞬时峰值。 NT4 mRNA在第IV和VI层中，提示在丘脑受体层中产生NT4蛋白，但峰值表达与NGF表达一样逐渐移至上层。神经营养蛋白mRNA的层特异性发育表达变化与形态发生过程相关。

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