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首页> 外文期刊>The European Journal of Neuroscience >Calcium-induced calcium release in rod photoreceptor terminals boosts synaptic transmission during maintained depolarization.
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Calcium-induced calcium release in rod photoreceptor terminals boosts synaptic transmission during maintained depolarization.

机译：杆状光感受器末端中钙诱导的钙释放增强了维持去极化过程中的突触传递。

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摘要

We examined the contribution of calcium-induced calcium release (CICR) to synaptic transmission from rod photoreceptor terminals. Whole-cell recording and confocal calcium imaging experiments were conducted on rods with intact synaptic terminals in a retinal slice preparation from salamander. Low concentrations of ryanodine stimulated calcium increases in rod terminals, consistent with the presence of ryanodine receptors. Application of strong depolarizing steps (-70 to -10 mV) exceeding 200 ms or longer in duration evoked a wave of calcium that spread across the synaptic terminals of voltage-clamped rods. This secondary calcium increase was blocked by high concentrations of ryanodine, indicating it was due to CICR. Ryanodine (50 microm) had no significant effect on rod calcium current (I(ca)) although it slightly diminished rod light-evoked voltage responses. Bath application of 50 microm ryanodine strongly inhibited light-evoked currents in horizontal cells. Whether applied extracellularly or delivered into the rod cell through the patch pipette, ryanodine (50 microm) also inhibited excitatory post-synaptic currents (EPSCs) evoked in horizontal cells by depolarizing steps applied to rods. Ryanodine caused a preferential reduction in the later portions of EPSCs evoked by depolarizing steps of 200 ms or longer. These results indicate that CICR enhances calcium increases in rod terminals evoked by sustained depolarization, which in turn acts to boost synaptic exocytosis from rods.
机译：我们检查了钙诱导的钙释放（CICR）对杆感光细胞末端突触传递的贡献。在sal的视网膜切片制备中，在具有完整突触末端的棒上进行了全细胞记录和共聚焦钙成像实验。低浓度的金刚烷胺刺激的钙在杆末端增加，与金刚烷胺受体的存在一致。持续时间超过200毫秒或更长时间的强力去极化步骤（-70至-10 mV）引起钙波，该钙波遍布电压钳制棒的突触末端。钙的这种二次增加被高浓度的莱诺碱阻止，表明这是由于CICR引起的。 Ryanodine（50微米）对棒状钙电流（I（ca））没有显着影响，尽管它略微减少了棒状光诱发的电压响应。在浴中使用50微米的ryanodine可强烈抑制水平细胞中的诱发电流。无论是在细胞外施用还是通过补片吸管递送至杆状细胞，ryanodine（50微米）还可以通过对杆进行去极化步骤来抑制水平细胞中诱发的兴奋性突触后电流（EPSC）。 Ryanodine通过200 ms或更长时间的去极化步骤引起了EPSC后期的优先减少。这些结果表明，CICR增强了由持续去极化引起的杆末端钙的增加，这反过来又促进了杆的突触胞吐作用。

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