A local GABAergic system within rat trigeminal ganglion cells.

Hayasaki H; Sohma Y; Kanbara K; Maemura K; Kubota T; Watanabe M

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A local GABAergic system within rat trigeminal ganglion cells.

机译：大鼠三叉神经节细胞内的局部GABA能系统。

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We investigated the GABAergic system within the Sprague-Dawley rat (2-3-weeks old) trigeminal ganglion (TG). Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed expression of glutamate decarboxylase (GAD) 65 and GAD67 mRNAs and mRNAs encoding GABA(A) receptor subunits alpha1-6, beta1-3, gamma1-3, and delta. In situ hybridization revealed that GAD65 and GAD67 mRNAs were expressed in neuronal cell bodies but not satellite cells. Immunohistochemical analysis showed that only GAD65 was expressed in all neuronal cell bodies, and approximately 70% of all neurons exhibited GABA immunoreactivity. Satellite cells were strongly immunopositive for GABA. GABA(A) receptor alpha1, alpha5, beta2/3 and gamma1/2/3 subunit immunoreactivities were observed in the majority of neurons, but no immunoreactivity for alpha2 was observed. Two types of cells were identified in TG based on cell size and morphology, type A and B. The percentage of cells expressing alpha3, alpha4, alpha6, and delta subunits appeared to be dependent on cell size, as delta and alpha6 expression were only observed in small (B-type) neurons. In whole-cell patch clamp experiments, GABA application induced inward Cl- currents in all neurons examined. The EC50 for GABA varied from 5.3 to 240 microm, and the Hill Coefficient (nH) varied between 0.98 and 2.6 at -60 mV. We found that GABA was released from TG cells by increasing extracellular K+ concentration to 100 mm. We speculate that GABA acts as a nonsynaptically released diffusible neurotransmitter, which may modulate somatic inhibition of neurons within the TG.

机译：我们调查了Sprague-Dawley大鼠（2-3周大）三叉神经节（TG）中的GABA能系统。逆转录聚合酶链反应（RT-PCR）分析显示谷氨酸脱羧酶（GAD）65和GAD67 mRNA和编码GABA（A）受体亚基alpha1-6，beta1-3，γ1-3和δ的mRNA的表达。原位杂交显示GAD65和GAD67 mRNA在神经元细胞体中表达，但在卫星细胞中不表达。免疫组织化学分析显示，在所有神经元细胞体中仅表达GAD65，并且所有神经元中约70％表现出GABA免疫反应性。卫星细胞对GABA具有强免疫阳性。在大多数神经元中均观察到GABA（A）受体alpha1，alpha5，beta2 / 3和gamma1 / 2/3亚基免疫反应性，但未观察到针对alpha2的免疫反应性。根据细胞大小和形态，TG中鉴定出两种类型的细胞，即A型和B型。表达alpha3，alpha4，alpha6和delta亚基的细胞百分比似乎取决于细胞大小，因为仅观察到了delta和alpha6表达在小（B型）神经元中。在全细胞膜片钳实验中，GABA的应用在所有检查的神经元中诱导了内向Cl电流。 GABA的EC50在5.3至240微米之间变化，希尔系数（nH）在-60 mV时在0.98至2.6之间变化。我们发现，通过将细胞外K +浓度增加到100 mm，GABA从TG细胞释放出来。我们推测，GABA充当非突触释放的可扩散神经递质，可能调节TG中神经元的体细胞抑制。

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《The European Journal of Neuroscience》 |2006年第3期|共13页
作者
Hayasaki H; Sohma Y; Kanbara K; Maemura K; Kubota T; Watanabe M;
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正文语种 eng
中图分类神经病学与精神病学;
关键词
gamma-Aminobutyric Acid; Cells; Triglycerides measurement; SIZES; 细胞;

机译：γ-氨基丁酸;细胞;甘油三酸酯测定;SIZES;细胞;

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1. A local GABAergic system within rat trigeminal ganglion cells. [J] . Hayasaki H, Sohma Y, Kanbara K, The European Journal of Neuroscience . 2006,第3期

机译：大鼠三叉神经节细胞内的局部GABA能系统。
2. Participation of the GABAergic system in the action of 2-amino-4-phosphonobutyrate on the OFF responses of frog retinal ganglion cells. [J] . Popova E, Mitova L, Vitanova L, Vision Research: An International Journal in Visual Science . 2003,第6期

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3. Immunohistochemical colocalization of TREK-1, TREK-2 and TRAAK with TRP channels in the trigeminal ganglion cells. [J] . Yamamoto Y, Hatakeyama T, Taniguchi K Neuroscience Letters: An International Multidisciplinary Journal Devoted to the Rapid Publication of Basic Research in the Brain Sciences . 2009,第2期

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6. Ultrastructural localization of cholinesterase activity in the trigeminal ganglion of the rat. [O] . J P Mazza, J S Hanker, A D Dixon 1973

机译：胆碱酯酶活性在大鼠三叉神经节中的超微结构定位。
7. Е. Holmgren. Note d. Spinal ganglion cells. (With. 11 images.). Anat. Vol. 16, № 7, p. 161-171. 1899. Idem. Further communications about d. Structure of the nerve cells. (With. 13 images.). Ibidem. Vol. 16. N 15/162 р. 388-397. 1899. F. Studnicka. About the occurrence of v. Canals u. Alveoli in the body d. Ganglion cells and in the axis cylinder of some nerve fibers, vol. 16. N 15/162 р. d. Vertebrates. Ibid. 397-401. 1899. Alb. Adamkiewicz. To the blood vessel system of the ganglion cell. Ibid. Vol. 17. N 2/3 p. 44. 1900. Е. Holmgren. Still other communications about the construction d. Nerve cells from various animals. (With. 17 image). Ibid. Vol. 17, N 6/7 p. 113 - 129. 1900. Idem. A few words on the occasion of the last communication published by Prof. Adamkiewicz. Ibidem, Vol. 17, N 15. 1900. A. Bethe. Some remarks on the “intracellular tubules” d spinal ganglion cells, etc. — Ibid. Vol. 17. N 16/17 p. 304-309 (with 3 images). 1900 [O] . A. Geberg 2020

机译：е..霍尔格伦。著名的。脊神经节细胞。（带有。11图像。）。 anat。卷。 16，№7，p。 161-171。 1899. idem。关于d的进一步通信。神经细胞的结构。（用。13个图像。）。 Ibidem。卷。 16. n 15/162р。 388-397。 1899. F. Studnicka。关于v的发生。运河u。肺泡在体内d。神经节细胞和一些神经纤维的轴筒，Vol。 16. n 15/162р。天。脊椎动物。同上。 397-401。 1899年。 Adamkiewicz。到神经节细胞的血管系统。同上。卷。 17. n 2/3 p。 44.1900.е。霍尔格伦。仍然有关施工D的其他通信。来自各种动物的神经细胞。（用。17图像）。同上。卷。 17，n 6/7 p。 113 - 129. 1900. idem。 Adamkiewicz教授发布的最后一次通信之际的几句话。 Ibidem，Vol。 17，n 15. 1900. A.贝特。关于“细胞内小管”D脊柱神经神经节细胞等的一些言论 - 同上。卷。 17. n 16/17 p。 304-309（有3张图片）。 1900

A local GABAergic system within rat trigeminal ganglion cells.

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