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首页> 外文期刊>The European Journal of Neuroscience >Apoptosis in the rat basal forebrain during development and following lesions of connections.
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Apoptosis in the rat basal forebrain during development and following lesions of connections.

机译：在发育过程中和连接损伤后大鼠基底前脑的细胞凋亡。

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Evidence suggests that neurotrophins are essential for the survival and phenotypic maintenance of cholinergic basal forebrain (BF) neurons. We evaluated the pattern of programmed cell death in the BF of the rat during development and after ablations of the cerebral cortex, a major target area and source of neurotrophins for BF neurons. We identified dying cells using the TUNEL (terminal deoxynucleotidyl-transferase-mediated dUTP-biotin nick end labelling) method and confirmed their apoptotic morphology with electron microscopy. Moreover, we demonstrated the expression of the apoptotic marker active caspase-3 in cells with features of apoptosis. TUNEL(+) cells were present in the developing BF during the first two postnatal weeks. Their frequency peaked at postnatal day (P)1 and at P5. TUNEL used in conjunction with immunofluorescence for neuronal nuclear protein (NeuN) showed that, at both peak stages, the majority of apoptotic cells were neurons. Extensive lesions of the cerebral cortex at different ages (P0, P7 and P14) did not induce significant changes in the frequency of apoptotic BF neurons. However, they resulted in alterations in the morphological phenotype of choline acetyltransferase (ChAT)-immunoreactive neurons in the BF, and a reduction in their number which was inversely proportional to the age at which the lesions were performed. We suggest that: (i) apoptosis is temporally coordinated with the morphological and neurochemical differentiation of BF neurons and the establishment of connections with their target areas; and (ii) cortical ablations do not affect the survival of BF neurons, but they influence the phenotype of cholinergic BF neurons.
机译：有证据表明，神经营养蛋白对于胆碱能基础前脑（BF）神经元的存活和表型维持至关重要。我们评估了发育过程中和大脑皮层消融后大鼠BF中程序性细胞死亡的模式，大脑皮层是BF神经元的主要靶标区域和神经营养蛋白的来源。我们使用TUNEL（末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记）方法鉴定了即将死亡的细胞，并通过电子显微镜确认了它们的凋亡形态。此外，我们证明了具有凋亡特征的细胞中凋亡标记物活性胱天蛋白酶3的表达。在出生后的前两周，发育中的BF中存在TUNEL（+）细胞。他们的频率在产后第1天和第5天达到峰值。 TUNEL与免疫荧光一起用于神经元核蛋白（NeuN）显示，在两个峰值阶段，大多数凋亡细胞都是神经元。不同年龄（P0，P7和P14）的大脑皮层的广泛病变并未引起凋亡性BF神经元频率的显着变化。但是，它们导致了BF中胆碱乙酰转移酶（ChAT）免疫反应性神经元的形态学表型改变，并且其数量减少与病变发生的年龄成反比。我们建议：（i）凋亡在时间上与BF神经元的形态和神经化学分化以及与它们的靶区域的连接的建立有关; （ii）皮质切除不影响BF神经元的存活，但会影响胆碱能BF神经元的表型。

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