...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Altered expression and functions of serotonin 5-HT1A and 5-HT1B receptors in knock-out mice lacking the 5-HT transporter.
【24h】

Altered expression and functions of serotonin 5-HT1A and 5-HT1B receptors in knock-out mice lacking the 5-HT transporter.

机译：在缺乏5-HT转运蛋白的基因敲除小鼠中，血清素5-HT1A和5-HT1B受体的表达和功能发生了改变。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

By taking up serotonin (5-hydroxytryptamine, 5-HT) released in the extracellular space, the 5-HT transporter (5-HTT) regulates central 5-HT neurotransmission. Possible adaptive changes in 5-HT neurotransmission in knock-out mice that do not express the 5-HT transporter were investigated with special focus on 5-HT1A and 5-HT1B receptors. Specific labelling with radioligands and antibodies, and competitive RT-PCR, showed that 5-HT1A receptor protein and mRNA levels were significantly decreased in the dorsal raphe nucleus (DRN), increased in the hippocampus and unchanged in other forebrain areas of 5-HTT-/- vs. 5-HTT+/+ mice. Such regional differences also concerned 5-HT1B receptors because a decrease in their density was found in the substantia nigra (-30%) but not the globus pallidus of mutant mice. Intermediate changes were noted in 5-HTT+/- mice compared with 5-HTT+/+ and 5-HTT-/- animals. Quantification of [35S]GTP-gamma-S binding evoked by potent 5-HT1 receptor agonists confirmed such changes as a decrease in this parameter was noted in the DRN (-66%) and the substantia nigra (-30%) but not other brain areas in 5-HTT-/- vs. 5-HTT+/+ mice. As expected from actions mediated by functional 5-HT1A and 5-HT1B autoreceptors, a decrease in brain 5-HT turnover rate after i.p. administration of ipsapirone (a 5-HT1A agonist), and an increased 5-HT outflow in the substantia nigra upon local application of GR 127935 (a 5-HT1B/1D antagonist) were observed in 5-HTT+/+ mice. Such effects were not detected in 5-HTT-/- mice, further confirming the occurrence of marked alterations of 5-HT1A and 5-HT1B autoreceptors in these animals.
机译：通过吸收5-羟色胺（5-羟色胺，5-HT）释放到细胞外空间，5-HT转运蛋白（5-HTT）调节中枢5-HT神经传递。研究了不表达5-HT转运蛋白的基因敲除小鼠中5-HT神经传递的可能适应性变化，特别关注了5-HT1A和5-HT1B受体。放射性配体和抗体的特异性标记以及竞争性RT-PCR表明，背根核（DRN）中的5-HT1A受体蛋白和mRNA水平显着降低，海马体中的5-HT1A受体蛋白和mRNA水平显着升高，而其他前脑区域中的5-HTT-与5-HTT + / +小鼠相比。这种区域差异也与5-HT1B受体有关，因为在黑质（-30％）中发现了它们密度的降低，而在突变小鼠的苍白球中却没有发现。与5-HTT + / +和5-HTT-/-动物相比，在5-HTT +/-小鼠中注意到中间变化。强大的5-HT1受体激动剂引起的[35S]GTP-γ-S结合的定量证实了这样的变化，如DRN（-66％）和黑质（-30％）中注意到该参数降低，但其他没有5-HTT-/-与5-HTT + / +小鼠的大脑区域。正如功能性5-HT1A和5-HT1B自身受体介导的作用所预期的那样，腹腔注射后脑部5-HT转换率降低。在5-HTT + / +小鼠中观察到，在局部应用GR 127935（5-HT1B / 1D拮抗剂）后，ipsapirone（5-HT1A激动剂）的给药和黑质中5-HT流出增加。在5-HTT-/-小鼠中未检测到这种作用，进一步证实了这些动物中5-HT1A和5-HT1B自体受体发生明显改变。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号