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首页> 外文期刊>The European Journal of Neuroscience >Identification of ventricular-side-enriched molecules regulated in a stage-dependent manner during cerebral cortical development.
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Identification of ventricular-side-enriched molecules regulated in a stage-dependent manner during cerebral cortical development.

机译：鉴定脑皮质发育过程中以阶段依赖性方式调节的脑室富集分子。

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Radial glial cells are the main component of the embryonic cortical ventricular zone (VZ), producing deep-layer excitatory neurons in the early stage and upper-layer excitatory neurons in the late stage of development. Previous studies have suggested that the laminar fate of deep-layer neurons might be determined by early-stage-specific secretory or transmembrane molecules (S/TMs) in the VZ. However, the different properties required to produce the different types of neurons in early-stage and late-stage VZ cells are largely unknown. Herein, we investigated the stage-dependent transcriptional profiles of the ventricular side of the mouse cortex, which was manually dissected at embryonic day (E)12, E14 and E16, and identified 3985 'VZ-enriched' genes, regulated stage-dependently, by GeneChip analysis. These molecules were classified into nine types based on stage-dependent regulation patterns. Prediction programs for the S/TMs revealed 659 'VZ-enriched' S/TMs. In situ hybridization and real-time PCR analysis for several of these molecules showed results consistent with the statistical analysis of the GeneChip experiments. Moreover, we identified 17 cell cycle-related early-stage and 'VZ-enriched' molecules. These molecules included not only those involved in cell cycle progression, but also essential molecules for DNA double-strand break repair, such as Rad51 and Rpa1. These results suggest that the early stage-VZ cells, which produce both deep- and upper-layer neurons, and the late-stage VZ cells, which produce only upper-layer neurons, are intrinsically different. The gene lists presented here will be useful for the investigation of stage-dependent changes in VZ cells and their regulatory mechanisms in the developing cortex.
机译：ial神经胶质细胞是胚胎皮质心室区（VZ）的主要成分，在发育的早期产生深层的兴奋性神经元，在发育的晚期产生上层的兴奋性神经元。先前的研究表明，深层神经元的层状命运可能由VZ中的早期特异性分泌或跨膜分子（S / TMs）决定。但是，在早期和晚期VZ细胞中产生不同类型的神经元所需的不同属性在很大程度上是未知的。在本文中，我们研究了小鼠皮质心室侧的阶段依赖性转录谱，该结构在胚胎第（E）12，E14和E16天进行了解剖，并鉴定了3985个“ VZ富集”基因，它们受阶段调控，通过GeneChip分析。根据阶段依赖性调节模式将这些分子分为九种类型。 S / TM的预测程序显示了659个“ VZ丰富”的S / TM。这些分子中的几种的原位杂交和实时PCR分析显示结果与GeneChip实验的统计分析一致。此外，我们确定了17个细胞周期相关的早期和“ VZ富集”分子。这些分子不仅包括那些参与细胞周期进程的分子，还包括DNA双链断裂修复所必需的分子，例如Rad51和Rpa1。这些结果表明，既产生深层神经元又产生上层神经元的早期VZ细胞与仅产生上层神经元的晚期VZ细胞本质上是不同的。此处介绍的基因列表将有助于研究VZ细胞的阶段依赖性变化及其在发育中的皮层中的调控机制。

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