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首页> 外文期刊>The European Journal of Neuroscience >Characterization of the glycinergic input to bipolar cells of the mouse retina.
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Characterization of the glycinergic input to bipolar cells of the mouse retina.

机译：小鼠视网膜双极细胞的甘氨酸输入信号的特征。

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Glycine and gamma-aminobutyric acid (GABA) are the major inhibitory transmitters of the mammalian retina, and bipolar cells receive GABAergic and glycinergic inhibition from multiple amacrine cell types. Here we evaluated the functional properties and subunit composition of glycine receptors (GlyRs) in bipolar cells. Patch-clamp recordings were performed from retinal slices of wild-type, GlyRalpha1-deficient (Glra1(spd-ot)) and GlyRalpha3-deficient (Glra3(-/-)) mice. Whole-cell currents following glycine application and spontaneous inhibitory postsynaptic currents (IPSCs) were analysed. During the recordings the cells were filled with Alexa 488 and, thus, unequivocally identified. Glycine-induced currents of bipolar cells were picrotoxinin-insensitive and thus represent heteromeric channels composed of alpha and beta subunits. Glycine-induced currents and IPSCs were absent from all bipolar cells of Glra1(spd-ot) mice, indicating that GlyRalpha1 is an essential subunit of bipolar cell GlyRs. By comparing IPSCs of bipolar cells in wild-type and Glra3(-/-) mice, no statistically significant differences were found. OFF-cone bipolar (CB) cells receive a strong glycinergic input from AII amacrine cells, that is preferentially based on the fast alpha1beta-containing channels (mean decay time constant tau = 5.9 +/- 1.4 ms). We did not observe glycinergic IPSCs in ON-CB cells and could elicit only small, if any, glycinergic currents. Rod bipolar cells receive a prominent glycinergic input that is mainly mediated by alpha1beta-containing channels (tau = 5.5 +/- 1.6 ms). Slow IPSCs, the characteristic of GlyRs containing the alpha2 subunit, were not observed in bipolar cells. Thus, different bipolar cell types receive kinetically fast glycinergic inputs, preferentially mediated by GlyRs composed of alpha1 and beta subunits.
机译：甘氨酸和γ-氨基丁酸（GABA）是哺乳动物视网膜的主要抑制性递质，双极细胞从多种无长素细胞类型中获得GABA能和甘氨酸能抑制作用。在这里，我们评估了双极细胞中甘氨酸受体（GlyRs）的功能特性和亚基组成。从野生型，GlyRalpha1缺陷型（Glra1（spd-ot））和GlyRalpha3缺陷型（Glra3（-/-））小鼠的视网膜切片进行膜片钳记录。分析了甘氨酸应用后的全细胞电流和自发抑制性突触后电流（IPSC）。在记录过程中，细胞中充满了Alexa 488，因此被明确标识。甘氨酸诱导的双极细胞电流对pict​​otoxinin不敏感，因此代表由α和β亚基组成的异聚通道。 Glra1（spd-ot）小鼠的所有双极细胞中都没有甘氨酸诱导的电流和IPSC，这表明GlyRalpha1是双极细胞GlyRs的必需亚基。通过比较野生型和Glra3（-/-）小鼠中双极细胞的IPSC，没有发现统计学上的显着差异。锥外双极（CB）细胞从AII无长春碱细胞接受强力的甘氨酸输入，这优先基于快速的含alpha1beta通道（平均衰减时间常数tau = 5.9 +/- 1.4 ms）。我们没有在ON-CB细胞中观察到甘氨酸能的IPSC，并且只能引起很小的甘氨酸能电流（如果有的话）。杆状双极细胞接受主要由包含alpha1beta的通道（tau = 5.5 +/- 1.6 ms）介导的突出的甘氨酸输入。在双极细胞中未观察到慢速IPSC，即含有α2亚基的GlyRs的特征。因此，不同的双极型细胞接受动力学上快速的甘氨酸输入，优先由由α1和β亚基组成的GlyR介导。

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