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首页> 外文期刊>The European Journal of Neuroscience >Sex differences in catechol contents in the olfactory bulb of control and unilaterally deprived rats.
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Sex differences in catechol contents in the olfactory bulb of control and unilaterally deprived rats.

机译：对照组和单侧剥夺大鼠嗅球中儿茶酚含量的性别差异。

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摘要

The dopaminergic system plays important roles in the modulation of olfactory transmission. The present study examines the distribution of dopaminergic cells and the content of dopamine (DA) and its metabolites in control and deprived olfactory bulbs (OB), focusing on the differences between sexes. The content of DA and of its metabolites, dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), were measured by HPLC. The morphology and distribution of dopaminergic neurons were studied using tyrosine hydroxylase (TH) immunohistochemistry. Cells were typified with TH-parvalbumin, TH-cholecystokinin or TH-neurocalcin double-immunofluorescence assays. Biochemical analyses revealed sex differences in the content of DA and of its metabolites. In normal conditions, the OBs of male rats had higher concentrations of DA, DOPAC and HVA than the OBs of females. The immunohistochemical data pointed to sex differences in the number of TH-immunopositive cells (higher in male than in female rats). Colocalization analyses revealed that dopaminergic cells constitute a different cell subpopulation from those labelled after parvalbumin, cholecystokinin or neurocalcin immunostaining. Unilateral olfactory deprivation caused dramatic alterations in the dopaminergic system. The DA content and the density of dopaminergic cells decreased, the contents of DA and DOPAC as well as TH immunoreactivity were similar in deprived males and females and, finally, the metaboliteeurotransmitter ratio increased. Our results show that the dopaminergic modulation of olfactory transmission seems to differ between males and females and that it is regulated by peripheral olfactory activity. A possible role of the dopaminergic system in the sexually different olfactory sensitivity, discrimination and memory is discussed.
机译：多巴胺能系统在嗅觉传递的调节中起重要作用。本研究研究了多巴胺能细胞的分布以及对照和剥夺性嗅球（OB）中多巴胺（DA）及其代谢产物的含量，重点是性别之间的差异。通过HPLC测量DA及其代谢产物二羟基苯基乙酸（DOPAC）和高香草酸（HVA）的含量。使用酪氨酸羟化酶（TH）免疫组织化学研究了多巴胺能神经元的形态和分布。用TH-小白蛋白，TH-胆囊收缩素或TH-神经钙蛋白双重免疫荧光测定法对细胞进行分类。生化分析表明，DA及其代谢产物的含量存在性别差异。在正常条件下，雄性大鼠的OB比雌性OB具有更高的DA，DOPAC和HVA浓度。免疫组化数据表明，TH免疫阳性细胞数量存在性别差异（雄性大鼠高于雌性大鼠）。共定位分析表明，多巴胺能细胞与小白蛋白，胆囊收缩素或神经钙蛋白免疫染色后标记的细胞组成不同的细胞亚群。单方面的嗅觉剥夺导致多巴胺能系统发生巨大变化。被剥夺的雄性和雌性中DA含量和多巴胺能细胞密度降低，DA和DOPAC含量以及TH免疫反应性相似，最后代谢物/神经递质比增加。我们的结果表明，嗅觉传递的多巴胺能调节在男性和女性之间似乎有所不同，并且受周围嗅觉活动的调节。讨论了多巴胺能系统在不同性别的嗅觉敏感性，辨别力和记忆力中的可能作用。

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