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首页> 外文期刊>The European Journal of Neuroscience >Unbiased cell quantification reveals a continued increase in the number of neocortical neurones during early post-natal development in mice.
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Unbiased cell quantification reveals a continued increase in the number of neocortical neurones during early post-natal development in mice.

机译：无偏见的细胞定量揭示了小鼠出生后早期发育过程中新皮层神经元数量的持续增加。

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The post-natal growth spurt of the mammalian neocortex has been attributed to maturation of dendritic arborizations, growth and myelination of axons, and addition of glia. It is unclear whether this growth may also involve recruitment of additional neurones. Using stereological methods, we analysed the number of neurones and glia in the neocortex during post-natal development in two separate strains of mice. Cell counting by the optical fractionator revealed that the number of neurones increased 80-100% from the time of birth to post-natal day (P)16, followed by a reduction by approximately 25% in the young adult mouse at P50-55. Unexpectedly, at the time of birth less than half of the neurones and at P8 only 65% of the neurones expressed neuronal nuclear antigen (NeuN), a marker of mature post-migratory neurones. In accordance with these observations, NeuN acquisition by neurones in layer VIa was delayed until P16. The number of glia reached its maximum at P16, whereas the number of oligodendroglia, identified using a transgenic marker, increased until P55, the latest time of observation. Neurones continued to accumulate in the developing neocortex during the first 2 weeks of post-natal development, underscoring fundamental differences in brain development in the mouse compared with human and non-human primates. Further, delayed acquisition of NeuN by neurones in the deepest neocortical layers and continued addition of oligodendroglia to the neocortex suggested that neocortical maturation should be regarded as an ongoing process continuing into the young adult mouse.
机译：哺乳动物新皮层的产后生长突增归因于树突状树突的成熟，轴突的生长和髓鞘化以及神经胶质的增加。目前尚不清楚这种增长是否还可能涉及补充其他神经元。使用立体学方法，我们分析了两个单独的小鼠品系在出生后发育过程中新皮层中神经元和神经胶质的数量。通过光学分馏仪进行的细胞计数显示，从出生到出生后的一天（P）16，神经元的数量增加了80-100％，然后在P50-55的成年小鼠中减少了约25％。出乎意料的是，在出生时，不到一半的神经元和P8处只有65％的神经元表达了神经元核抗原（NeuN），这是成熟的迁移后神经元的标志。根据这些观察，第VIa层中神经元的NeuN采集被延迟到P16。胶质细胞的数量在P16达到最大，而使用转基因标记鉴定的少突胶质细胞的数量增加，直到最近一次观察到的P55。在产后发育的前两周，神经元继续积聚在发育中的新皮层中，强调了与人类和非人类灵长类动物相比，小鼠大脑发育的根本差异。此外，神经元在最深的新皮层中延迟获取NeuN并向新皮层中继续添加少突胶质细胞表明，新皮层成熟应视为持续到成年小鼠的一个持续过程。

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