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首页> 外文期刊>The European Journal of Neuroscience >Reduction of metabotropic glutamate receptor-mediated heterosynaptic inhibition of developing MNTB-LSO inhibitory synapses.
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Reduction of metabotropic glutamate receptor-mediated heterosynaptic inhibition of developing MNTB-LSO inhibitory synapses.

机译：减少代谢型谷氨酸受体介导的对发育中的MNTB-LSO抑制性突触的异突触抑制。

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摘要

The lateral superior olivary nucleus (LSO) is an auditory relay centre within the brain stem that encodes interaural level differences for sound localization by integrating GABA/glycinergic input from the contralateral ear via the medial nucleus of the trapezoid body (MNTB), and glutamatergic input from the ipsilateral ear via the ventral cochlear nucleus (VCN). To study the development of the circuits that contribute to the establishment of sound localization, the heterosynaptic modulation mediated by glutamate released from VCN terminals and group II metabotropic glutamate receptor (mGluR) expressed on MNTB inhibitory terminals was investigated using whole-cell patch-clamp techniques. At postnatal day-4-8 (P4-8), repetitive stimulation of the VCN-LSO excitatory afferents caused significant inhibition of MNTB-LSO inhibitory postsynaptic currents (IPSCs) in amplitude with an increase of its coefficient of variation and changed the paired-pulse ratio. These effects were antagonized by LY341495, an mGluR2/3 antagonist. Thus, the suppression of MNTB-LSO synaptic responses induced by repetitive stimulation applied to the VCN-LSO glutamatergic afferent is presumably due to an activation of mGluR2/3 existing on MNTB-LSO presynaptic terminals. The suppression rate of MNTB-LSO IPSCs by DCG IV, an mGluR2/3 agonist, decreased with development and became negligible by the third week after birth. The immunohistochemical staining of mGluR2/3 in the LSO was also less apparent at P18 compared with that at P4. We suggest that mGluR-mediated heterosynaptic modulation of MNTB-LSO GABAergic/glycinergic transmission might contribute to the development of appropriate adult auditory circuits.
机译：外侧上橄榄核（LSO）是脑干内的听觉中继中心，通过整合来自对侧耳的GABA /糖能输入（通过梯形体的内侧核（MNTB））和谷氨酸能输入，对听觉水平差异进行声音定位从同侧耳经腹侧耳蜗核（VCN）。为了研究有助于建立声音定位的电路的发展，使用全细胞膜片钳技术研究了从VCN末端释放的谷氨酸和在MNTB抑制性末端表达的II型代谢型谷氨酸受体（mGluR）介导的异突触调节。 。在出生后的第4-8天（P4-8），重复刺激VCN-LSO兴奋性传入神经导致MNTB-LSO抑制性突触后电流（IPSC）振幅受到明显抑制，其变异系数增加，并改变了配对的脉冲比。这些作用被mGluR2 / 3拮抗剂LY341495拮抗。因此，推测由重复刺激施加到VCN-LSO谷氨酸能传入所诱导的MNTB-LSO突触反应的抑制可能是由于存在于MNTB-LSO突触前末端的mGluR2 / 3的激活。 DCG IV（一种mGluR2 / 3激动剂）对MNTB-LSO IPSC的抑制率随发育而降低，到出生后第三周可忽略不计。与P4相比，L18中的mGluR2 / 3的免疫组织化学染色在P18也较不明显。我们建议，mGluR介导的MNTB-LSO GABA能/甘氨酸能传递的异突触调节可能有助于适当的成人听觉回路的发展。

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