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首页> 外文期刊>The European Journal of Neuroscience >Sex differences in adult cell proliferation within the zebrafish (Danio rerio) cerebellum.
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Sex differences in adult cell proliferation within the zebrafish (Danio rerio) cerebellum.

机译：斑马鱼（Danio rerio）小脑内成年细胞增殖的性别差异。

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It has been reported that neurons generated in the adult brain show sex-specific differences in several brain regions of lower vertebrates and mammals. The present study questioned whether cell proliferation and survival in the adult zebrafish (Danio rerio) cerebellum, the most mitotically active area of adult teleost brain, is sexually differentiated. Adult zebrafish were treated with the thymidine analogue 5'-bromo-2'-deoxyuridine (BrdU) and allowed to survive for 24 h (short-term) and for 21 days (long-term). BrdU immunohistochemistry allowed visualization of cells incorporating BrdU at the S phase of mitosis. At short-term survival, male zebrafish had a higher number of labelled cells at proliferation sites of the molecular layer of corpus cerebelli (CCe) and the granular layer of the caudal lobe of the cerebellum (LCa) than did females. In long-term survival, BrdU-positive cells were found at their final destination, but only the granular layer of the medial division of the valvula cerebelli showed sex-specific differences in the number of labelled cells. This higher mitotic activity in male cerebellum might be related to sex-specific motor behaviour observed in male zebrafish. To investigate the role of programmed cell death, the terminal deoxynucleotidyl-mediated dUTP nick-end-labelling (TUNEL) method was applied. The vast majority of apoptotic figures occurred in the granular cell layer of valvula and CCe, only in a few cases within the BrdU-retaining cells. Apoptosis was found specifically at the sites of the final destination of proliferating cells, indicating that the close relation of cell birth and death might represent a possible plasticity mechanism in the adult zebrafish cerebellum.
机译：据报道，成年大脑中产生的神经元在低等脊椎动物和哺乳动物的几个大脑区域中表现出性别特异性差异。本研究质疑成年斑马鱼（成年硬脑膜最有丝分裂的活动区域）成年斑马鱼（达尼奥里奥）小脑的细胞增殖和存活是否存在性别差异。成年斑马鱼用胸苷类似物5'-溴-2'-脱氧尿苷（BrdU）处理，可以存活24小时（短期）和21天（长期）。 BrdU免疫组织化学可以观察到在有丝分裂的S期掺入BrdU的细胞。在短期存活时，与雌性相比，雄性斑马鱼在小脑）（CCe）分子层和小脑尾叶（LCa）的颗粒层的增殖位点具有更高数量的标记细胞。在长期存活中，BrdU阳性细胞被发现在它们的最终目的地，但是只有小脑谷内侧分裂的颗粒层在标记细胞的数量上表现出性别特异性差异。雄性小脑中较高的有丝分裂活性可能与雄性斑马鱼中观察到的性别特定运动行为有关。为了研究程序性细胞死亡的作用，应用了末端脱氧核苷酸介导的dUTP缺口末端标记（TUNEL）方法。绝大多数凋亡数字发生在瓣膜和CCe的颗粒细胞层中，仅在少数情况下发生在BrdU保留细胞内。细胞凋亡特别是在增殖细胞最终到达的位置发现，表明细胞生与死的密切关系可能代表了成年斑马鱼小脑的可能可塑性机制。

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