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首页> 外文期刊>The European Journal of Neuroscience >Emergence of topography in the developing hamster retinocollicular projection: axial differences and the role of cell death.
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Emergence of topography in the developing hamster retinocollicular projection: axial differences and the role of cell death.

机译：发育中的仓鼠视网膜胶状体投影中地形的出现：轴向差异和细胞死亡的作用。

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The precise ordering of the hamster retinocollicular projection is established over the first two postnatal weeks, coincident with developmental cell death. We have used quantitative retrograde labelling to define topographic precision in the early postnatal projection, to describe its refinement and to assess the contribution played by selective retinal ganglion cell death. The hamster's short gestation period allows the investigation of events occurring prenatally in other rodents. Discrete injections of fluorescent beads in the superior colliculus followed by isodensity contour analysis of labelled retinal cells reveals a dramatic decrease in the extent of retina labelled between postnatal days 2, 6 and 12 (P2, P6, P12): the 20% contour encloses 38.3%, 8.3% and 1.8% of the retina at these ages. Paired injections of two different tracers at variable rostrocaudal (R-C) separations at P2 produced complete overlap of label even when injections were separated by over 1 mm. This was not true for paired mediolateral injections at P2 that were separated by more than 500 microm. Analysis of the segregation of the two tracers ('nearest-neighbour analysis') shows topography improving with age so that by P12 injections separated rostrocaudally by more than 500 microm produced no overlap in the retina. To examine the contribution of selective ganglion cell death to topographic refinement, animals given paired R-C injections at P2 were allowed to survive until P12. Nearest-neighbour analysis reveals significantly more order in the P2-P12 retinae than after overnight survival. Thus, selective cell death plays a small but appreciable role in correction of topographical errors.
机译：仓鼠视网膜胶体投射的精确顺序是在出生后的前两周建立的，与发育性细胞死亡相吻合。我们已经使用定量逆行标记来定义产后早期投影中的地形精确度，以描述其精细化并评估选择性视网膜神经节细胞死亡所起的作用。仓鼠的妊娠期短，可以调查其他啮齿动物产前发生的事件。在上丘中离散注射荧光珠，然后对标记的视网膜细胞进行等渗轮廓分析，发现出生后第2、6和12天（P2，P6，P12）之间标记的视网膜范围显着减少：20％轮廓包围了38.3在这些年龄段，分别占视网膜的％，8.3％和1.8％。在P2处以可变的杆状尾（R-C）间距进行配对的两种不同示踪剂的配对进样，即使进样间隔超过1 mm，也可以完全重叠标记。对于在P2处相隔500微米以上的成对的中外侧注射，情况并非如此。对两种示踪剂的隔离分析（“最近邻分析”）显示，地形随着年龄的增长而改善，因此，通过P12注射，向后尾骨分开超过500微米，在视网膜上没有重叠。为了检查选择性神经节细胞死亡对地形细化的贡献，允许在P2进行配对R-C注射的动物存活到P12。最近邻居分析显示，P2-P12视网膜中的顺序比过夜生存后要多得多。因此，选择性细胞死亡在纠正地形误差中起着很小但可观的作用。

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