...
首页> 外文期刊>The European physical journal, E. Soft matter >Wormlike lipid/DNA micelles in a non-polar solvent
【24h】

Wormlike lipid/DNA micelles in a non-polar solvent

机译:非极性溶剂中的蠕虫样脂质/ DNA胶束

获取原文
获取原文并翻译 | 示例
           

摘要

The phase behavior of DOPE/DOTAP-DNA complexes in phase-separated oil(dodecane)/water mixtures was explored using Small Angle X-Ray Scattering (SAXS) and Fluorescence Correlation Spectroscopy (FCS). Inverse micelles of DNA with cationic-lipid coating were found in the oil phase. Varying the ratio between cationic and neutral lipids a transition from wormlike to spherical structures is observed for both long (approximate to 75000bp) and short (30-1246bp) DNA. In contrast to lipid/DNA complexes in the water phase, there is no indication of condensed liquid-crystalline structures in the non-polar phase. In fact, FCS measurements on short DNA oligomers complexed with cationic lipid in alkane give clear evidence for monomeric inverse micelles of DNA. Dilution series revealed a critical lower concentration of lipids and DNA for observing lipid/DNA micelles.
机译:使用小角X射线散射(SAXS)和荧光相关光谱(FCS)探索了DOPE / DOTAP-DNA复合物在相分离的油(十二烷)/水混合物中的相行为。在油相中发现了带有阳离子脂质涂层的DNA反胶束。改变阳离子脂质和中性脂质之间的比例,可以观察到长(约75000bp)和短(30-1246bp)DNA从蠕虫样向球形结构的转变。与水相中的脂质/ DNA复合物相反,没有迹象表明在非极性相中液晶结构会凝结。实际上,在烷烃中与阳离子脂质络合的短DNA低聚物的FCS测量提供了DNA单体反胶束的明确证据。稀释系列显示,用于观察脂质/ DNA胶束的脂质和DNA浓度较低。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号