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首页> 外文期刊>The European physical journal, E. Soft matter >The development of anisotropic behaviours of 3T3 fibroblasts on microgrooved patterns
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The development of anisotropic behaviours of 3T3 fibroblasts on microgrooved patterns

机译：3T3成纤维细胞在微槽纹上的各向异性行为的发展

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摘要

3T3 fibroblasts cultured on microgrooved polydimethylsiloxane (PDMS) surfaces of two different widths (25 μm and 55μm) were individually tracked using confocal microscopy with a novel live-cell staining technique over several hours without noticeable cytotoxic effects. By quantifying the cell morphology, orientation, and migration over time, we identified the timescale (about 2-4 h after seeding) over which cell behaviours transitioned from isotropy to anisotropy, where the preference is in the direction parallel to the pattern. The development of anisotropy occurred more rapidly and distinctly when a narrower ridge width was used, suggesting that it is the ridge width that imposed a physical barrier on the cells' morphology and motility. Furthermore, while we found a weak but statistically significant correlation between cell orientation and morphology on the single-cell level, there is a lack of correlation on the same level between cell orientation and migratory direction. This suggests that while morphology and migration are affected anisotropically by topographical patterns in a similar way, the underlying processes giving rise to the anisotropy is slightly different in the two cases.
机译：使用共聚焦显微镜和新颖的活细胞染色技术，在几个小时内分别跟踪在两个不同宽度（25μm和55μm）的微沟槽聚二甲基硅氧烷（PDMS）表面上培养的3T3成纤维细胞，没有明显的细胞毒性作用。通过量化细胞形态，方向和随时间的迁移，我们确定了细胞行为从各向同性转变为各向异性的时间尺度（接种后约2-4小时），其中偏爱方向平行于模式。当使用较窄的山脊宽度时，各向异性的发展会更快，更明显地发生，这表明正是山脊宽度对细胞的形态和运动施加了物理障碍。此外，虽然我们在单细胞水平上发现了细胞方向和形态之间的微弱但统计学上显着的相关性，但是在相同水平上的细胞方向和迁移方向之间却缺乏相关性。这表明尽管形态学和迁移受到地形图样各向异性的影响，但在两种情况下引起各向异性的基本过程却略有不同。

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