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首页> 外文期刊>The East African medical journal >Haematoxylin and eosin staining in the diagnosis of Hodgkin's disease in Uganda.
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Haematoxylin and eosin staining in the diagnosis of Hodgkin's disease in Uganda.

机译：苏木精和曙红染色在乌干达霍奇金氏病的诊断中。

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BACKGROUND: Whereas immunohistochemical methods have been widely used for the diagnosis and classification of Hodgkin's disease in the developed countries, there are very few reports of their use in the developing countries where haematoxylin and eosin is the mainstay of diagnosis of Hodgkin's disease. Yet the diagnostic accuracy of haematoxylin and eosin has not been assessed in Uganda. OBJECTIVE: To determine the reliability of haematoxylin and eosin staining in the diagnosis of Hodgkin's disease using immunohistochemistry as the reference standard. DESIGN: Laboratory based cross sectional study. SETTING: Makerere University Medical School, Department of Pathology. METHODOLOGY: Two hundred and forty formalin fixed, paraffin embedded biopsies seen in the Makerere University, Department of Pathology from 1980-2000 were studied. The tissue sections, were assessed and subjected to immunohistochemical methods using monoclonal antibodies including leucocyte common antigen, LCA (CD45), antibodies to Reed-Sternberg cells (CD15, CD30) and antibodies to B cells (CD20). The sensitivity, specificity, positive predictive value and negative predictive value were assessed. The overall Kappa score was used to assess the agreement between the two diagnostic tests. RESULTS: Of the 240 biopsies, 171(71.3%) were confirmed as Hodgkin's disease by immunohistochemistry. Using haematoxylin and eosin (H&E), only 131 of the 171 cases of Hodgkin's disease were detected. The mean age of the 171 cases was 26.1 (SD 16.2) years, with a mode of 20.0 and median of 22.5 years. The 15-24 year age group was the most affected (47.2%). There were more males (65.9%) than females and most were Baganda the dominant tribe in the central region. The sensitivity, specificity, positive and negative predictive values of haematoxylin and eosin were 76.61%, 92.75%, 96.32% and 61.53% respectively. The agreement between the two tests was 81.25% with an overall measure of agreement, Kappa, of 0.602. CONCLUSION: Haematoxylin and eosin has relatively high efficacy in the diagnosis of Hodgkin's disease. Use of haematoxylin and eosin is still recommended for the diagnosis of Hodgkin's disease, reserving the expensive immunohistochemistry for difficult cases.
机译：背景：尽管免疫组织化学方法在发达国家已广泛用于霍奇金病的诊断和分类，但在苏木精和曙红是诊断霍奇金病的主要手段的发展中国家中，很少有报道使用它们。尚未在乌干达评估苏木精和曙红的诊断准确性。目的：以免疫组织化学为参考标准，确定苏木精和曙红染色在霍奇金病诊断中的可靠性。设计：基于实验室的横截面研究。地点：马克雷雷大学医学院病理学系。方法：对1980-2000年在马凯雷雷大学病理学系进行的244次福尔马林固定，石蜡包埋的活组织检查进行了研究。使用单克隆抗体包括白细胞共同抗原，LCA（CD45），Reed-Sternberg细胞抗体（CD15，CD30）和B细胞抗体（CD20）评估组织切片并进行免疫组织化学方法免疫。评估敏感性，特异性，阳性预测值和阴性预测值。总Kappa分数用于评估两次诊断测试之间的一致性。结果：240例活检组织中，有171例（占71.3％）被免疫组织化学证实为霍奇金氏病。使用苏木精和曙红（H＆E），在171例霍奇金病中仅发现131例。 171例患者的平均年龄为26.1（SD 16.2）岁，模式为20.0，中位数为22.5岁。 15-24岁年龄组受影响最大（47.2％）。男性（65.9％）多于女性，并且多数人是中部地区的支配部落巴甘达。苏木精和曙红的敏感性，特异性，阳性和阴性预测值分别为76.61％，92.75％，96.32％和61.53％。两次测试之间的一致性为81.25％，总体一致性度量Kappa为0.602。结论：苏木精和曙红在霍奇金病的诊断中具有较高的疗效。仍建议使用苏木精和曙红来诊断霍奇金氏病，从而在困难情况下保留昂贵的免疫组织化学。

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