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首页> 外文期刊>The FEBS journal >Planarian peptidylglycine-hydroxylating monooxygenase, a neuropeptide processing enzyme, colocalizes with cytochrome b(561) along the central nervous system
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Planarian peptidylglycine-hydroxylating monooxygenase, a neuropeptide processing enzyme, colocalizes with cytochrome b(561) along the central nervous system

机译：平面肽肽基甘氨酸羟化单加氧酶，一种神经肽加工酶，与细胞色素b（561）沿中枢神经系统共定位

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摘要

Planarians are one of the simplest animal groups with a central nervous system. Their primitive central nervous system produces large quantities of a variety of neuropeptides, of which many are amidated at their C terminus. In vertebrates, peptide amidation is catalyzed by two enzymes [peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxylglycine alpha-amidating lyase] acting sequentially. In mammals, both enzymatic activities are contained within a single protein that is encoded by a single gene. By utilizing PCR with degenerate oligonucleotides derived from conserved regions of PHM, we succeeded in cloning a full-length cDNA encoding planarian PHM. The deduced amino acid sequence showed full conservation of five His residues and one Met residue, which bind two Cu atoms that are essential for the activity of PHM. Northern blot analysis confirmed the expression of a PHM mRNA of the expected size. Distribution of the mRNA was analyzed by in situ hybridization, showing specific expression in neurons with two morphologically distinct structures, a pair of the ventral nerve cords and the brain. The distribution of PHM was very similar to that of cytochrome b(561). This indicates that the ascorbate-related electron transfer system operates in the planarian central nervous system to support the PHM activity and that it predates the emergence of Plathelminthes in the evolutionary history.
机译：平面动物是具有中枢神经系统的最简单的动物群体之一。他们原始的中枢神经系统会产生大量的各种神经肽，其中许多在其C端酰胺化。在脊椎动物中，顺序发生作用的两种酶[肽基甘氨酸α-羟基化单加氧酶（PHM）和肽基-α-羟基甘氨酸α-酰胺化裂解酶]催化肽酰胺化。在哺乳动物中，两种酶活性都包含在由单个基因编码的单个蛋白质中。通过利用PCR衍生自PHM保守区的简并寡核苷酸，我们成功克隆了编码涡虫PHM的全长cDNA。推导的氨基酸序列显示5个His残基和1个Met残基完全保守，它们结合了2个对PHM活性至关重要的Cu原子。 Northern印迹分析证实了预期大小的PHM mRNA的表达。通过原位杂交分析了mRNA的分布，显示了在具有两个形态上不同的结构，一对腹神经索和大脑的神经元中的特异性表达。 PHM的分布与细胞色素b（561）非常相似。这表明与抗坏血酸有关的电子转移系统在涡虫中枢神经系统中起作用，以支持PHM活性，并且它在进化历史中早于鼠疫的出现。

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