...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biomaterials Science >Silica as a morphogenetically active inorganic polymer
【24h】

Silica as a morphogenetically active inorganic polymer

机译：二氧化硅作为形态发生活性的无机聚合物

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

At present the scaffolds used for bioprinting of cells do not elicit morphogenetic responses in the cells. In the present study we approached a solution by studying the effect of an inorganic silica supplement added to an Na-alginate matrix. Bone- and osteoblast-like SaOS-2 cells were embedded into this organic polymeric matrix which was additionally enriched with 400 μM prehydrolyzed TEOS [tetra-ethoxy-silane], a source of ortho-silicate. In this silica-based matrix the cells synthesized hydroxyapatite crystallites after exposure to a mineralization activation cocktail composed of β-glycerophosphate, ascorbic acid and dexamethasone. The degree of hydroxyapatite synthesis, determined by staining the cells with the Osteo-Image dye, strongly increased after exposure of the cells to silica. In a previous study we reported that ortho-silicate induces the expression of the gene encoding BMP-2 [bone morphogenetic protein-2]. Now we asked the question whether, in the presence of the mineralization activation cocktail, silica induces differentially the fibrillar proteins type I collagen [COLI] and type V collagen [COLV], as well as the non-collagenous proteins alkaline phosphatase [ALP], osteopontin [OPN], osteonectin [ON], osteocalcin [OC], and bone sialoprotein II [BSP]. Those expression values were correlated with the transcript levels of RUNX2 [Runt-related transcription factor 2]. The data show that the steady-state transcript level of RUNX2 remained unchanged in the presence of silica, while this inorganic polymer caused an elevated BMP-2 transcript level, and simultaneously also a significant upregulation of the COLI, COLV, OPN and ON genes. In contrast, the level of expression of OC and BSP remained unchanged in the presence of silica. It is concluded that silica causes its morphogenetic effect with respect to some bone-specific genes, COLI, COLV, OPN and ON, in a RUNX2-independent way.
机译：目前，用于细胞生物打印的支架尚未引起细胞的形态发生反应。在本研究中，我们通过研究将无机二氧化硅补充剂添加到海藻酸钠基体中的作用来寻求解决方案。将类骨和成骨细胞样的SaOS-2细胞嵌入到该有机聚合物基质中，该基质还富含400μM预水解的TEOS [四乙氧基硅烷]（原硅酸盐来源）。在这种基于二氧化硅的基质中，细胞暴露于由β-甘油磷酸，抗坏血酸和地塞米松组成的矿化活化混合物后，可合成羟基磷灰石微晶。通过将细胞用Osteo-Image染料染色确定的羟磷灰石合成度，在细胞暴露于二氧化硅后会大大增加。在先前的研究中，我们报道了原硅酸盐诱导了BMP-2编码基因的表达[骨形态发生蛋白2]。现在我们问一个问题，在存在矿化活化混合物的情况下，二氧化硅是否会差异诱导纤维蛋白I型胶原[COLI]和V型胶原[COLV]以及非胶原蛋白碱性磷酸酶[ALP]骨桥蛋白[OPN]，骨连接蛋白[ON]，骨钙蛋白[OC]和骨唾液蛋白II [BSP]。这些表达值与RUNX2 [Runt相关转录因子2]的转录水平相关。数据显示，在存在二氧化硅的情况下，RUNX2的稳态转录水平保持不变，而这种无机聚合物导致BMP-2转录水平升高，并且同时还显着上调了COLI，COLV，OPN和ON基因。相反，在二氧化硅存在下，OC和BSP的表达水平保持不变。结论是，二氧化硅以RUNX2无关的方式引起了其对某些骨特异性基因COLI，COLV，OPN和ON的形态发生作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号