...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The FEBS journal >Defining the genetic relationship of protegrin-related sequences and the in vivo expression of protegrins
【24h】

Defining the genetic relationship of protegrin-related sequences and the in vivo expression of protegrins

机译：定义protegrin相关序列的遗传关系和protegrins的体内表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Protegrins (PGs) are potent antimicrobial peptides that act on a broad spectrum of microorganisms, including bacteria, fungi and some enveloped viruses. We analyzed the expression pattern of protegrins in 17 different pig tissues using RT-PCR, and developed an anti-(PG-1) polyclonal IgG. Western blot analysis using the antibody showed that protegrins are mainly present as prepropeptide forms in normal tissues, rather than as mature peptides. Immunohistochemical analysis showed that protegrin expression was specific to a few cell types, including neutrophils, pulmonary club, epithelial and Leydig cells. Genetic analyses of the five previously reported protegrin sequences showed that they are encoded at a single locus, rather than from multiple paralogous genes. By genotyping 28 animals across five breeds, we identified eight different alleles of the PGs.
机译：Protegrins（PGs）是有效的抗菌肽，可作用于广泛的微生物，包括细菌，真菌和某些包膜病毒。我们使用RT-PCR分析了protegrins在17种不同猪组织中的表达模式，并开发了抗（PG-1）多克隆IgG。使用该抗体的蛋白质印迹分析表明，蛋白凝集素主要以原肽形式存在于正常组织中，而不是以成熟的肽形式存在。免疫组织化学分析表明，protegrin表达对几种细胞具有特异性，包括嗜中性粒细胞，肺部俱乐部，上皮细胞和Leydig细胞。对五个以前报道的protegrin序列的遗传分析表明，它们是在一个基因座编码的，而不是来自多个旁系同源基因。通过对五个品种的28只动物进行基因分型，我们确定了PG的八个等位基因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号