...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The FEBS journal >Spectroscopic characterization of the oxyferrous complex of prostacyclin synthase in solution and in trapped sol-gel matrix
【24h】

Spectroscopic characterization of the oxyferrous complex of prostacyclin synthase in solution and in trapped sol-gel matrix

机译：溶液和捕集的溶胶-凝胶基质中前列环素合酶的氧化亚铁配合物的光谱表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Prostacyclin synthase (PGIS) is a member of the cytochrome P450 family in which the oxyferrous complexes are generally labile in the absence of substrate. At 4 degrees C, the on-rate constants and off-rate constants of oxygen binding to PGIS in solution are 5.9 x 10(5) M-1.s(-1) and 29 s(-1), respectively. The oxyferrous complex decays to a ferric form at a rate of 12 s(-1). We report, for the first time, a stable oxyferrous complex of PGIS in a transparent sol-gel monolith. The encapsulated ferric PGIS retained the same spectroscopic features as in solution. The binding capabilities of the encapsulated PGIS were demonstrated by spectral changes upon the addition of O-based, N-based and C-based ligands. The peroxidase activity of PGIS in sol-gel was three orders of magnitude slower than that in solution owing to the restricted diffusion of the substrate in sol-gel. The oxyferrous complex in sol-gel was observable for 24 h at room temperature and displayed a much red-shifted Soret peak. Stabilization of the ferrous-carbon monoxide complex in sol-gel was observed as an enrichment of the 450-nm species over the 420-nm species. This result suggests that the sol-gel method may be applied to other P450s to generate a stable intermediate in the di-oxygen activation.
机译：前列环素合酶（PGIS）是细胞色素P450家族的成员，其中的氧化亚铁络合物在没有底物的情况下通常不稳定。在4摄氏度下，溶液中与PGIS结合的氧的开通速率常数和关断速率常数分别为5.9 x 10（5）M-1.s（-1）和29 s（-1）。氧化亚铁络合物以12 s（-1）的速率分解为三价铁。我们首次报告了透明溶胶-凝胶整体料中稳定的PGIS氧化亚铁络合物。封装的三价铁PGIS保留了与溶液相同的光谱特征。封装的PGIS的结合能力通过添加基于O的，基于N的和基于C的配体的光谱变化证明。由于底物在溶胶-凝胶中的限制扩散，溶胶-凝胶中PGIS的过氧化物酶活性比溶液中的过氧化物酶活性慢三个数量级。溶胶-凝胶中的氧化亚铁络合物在室温下可观察24小时，并显示出许多红移的Soret峰。观察到溶胶-凝胶中亚铁-一氧化碳络合物的稳定化，是因为450-nm物种比420-nm物种富集。该结果表明，溶胶-凝胶法可以应用于其他P450，以在双氧活化中生成稳定的中间体。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号