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首页> 外文期刊>The FEBS journal >Complete high-density lipoproteins in nanoparticle corona
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Complete high-density lipoproteins in nanoparticle corona

机译：纳米电晕中完整的高密度脂蛋白

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摘要

In a biological environment, nanoparticles immediately become covered by an evolving corona of biomolecules, which gives a biological identity to the nanoparticle and determines its biological impact and fate. Previous efforts at describing the corona have concerned only its protein content. Here, for the first time, we show, using size exclusion chromatography, NMR, and pull-down experiments, that copolymer nanoparticles bind cholesterol, triglycerides and phospholipids from human plasma, and that the binding reaches saturation. The lipid and protein binding patterns correspond closely with the composition of high-density lipoprotein (HDL). By using fractionated lipoproteins, we show that HDL binds to copolymer nanoparticles with much higher specificity than other lipoproteins, probably mediated by apolipoprotein A-I. Together with the previously identified protein binding patterns in the corona, our results imply that copolymer nanoparticles bind complete HDL complexes, and may be recognized by living systems as HDL complexes, opening up these transport pathways to nanoparticles. Apolipoproteins have been identified as binding to many other nanoparticles, suggesting that lipid and lipoprotein binding is a general feature of nanoparticles under physiological conditions.
机译：在生物环境中，纳米颗粒会立即被不断发展的生物分子电晕所覆盖，从而赋予纳米颗粒以生物学身份，并确定其生物学影响和命运。先前描述电晕的努力仅涉及其蛋白质含量。在这里，我们首次使用尺寸排阻色谱，NMR和下拉实验表明，共聚物纳米颗粒结合了人血浆中的胆固醇，甘油三酸酯和磷脂，并且结合达到饱和。脂质和蛋白质的结合模式与高密度脂蛋白（HDL）的组成密切相关。通过使用分级脂蛋白，我们显示HDL以比其他脂蛋白更高的特异性结合到共聚物纳米颗粒上，这可能是由载脂蛋白A-1介导的。连同先前在电晕中确定的蛋白质结合模式一起，我们的结果表明共聚物纳米颗粒结合了完整的HDL复合物，并可能被生命系统识别为HDL复合物，从而打开了这些向纳米颗粒的运输途径。载脂蛋白已被鉴定为与许多其他纳米颗粒结合，这表明脂质和脂蛋白结合是纳米颗粒在生理条件下的普遍特征。

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