Proton-decoupled N-15 and P-31 solid-state NMR investigations of the Pf3 coat protein in oriented phospholipid bilayers

Aisenbrey C; Harzer U; Bauer-Manz G; Bar G; Chotimah INH; Bertani P; Sizun C; Kuhn A; Bechinger B

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Proton-decoupled N-15 and P-31 solid-state NMR investigations of the Pf3 coat protein in oriented phospholipid bilayers

机译：定向磷脂双层中Pf3外壳蛋白的质子解耦N-15和P-31固态NMR研究

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The coat proteins of filamentous phage are first synthesized as transmembrane proteins and then assembled onto the extruding viral particles. We investigated the transmembrane conformation of the Pseudomonas aeruginosa Pf3 phage coat protein using proton-decoupled N-15 and P-31 solid-state NMR spectroscopy. The protein was either biochemically purified and uniformly labelled with N-15 or synthesized chemically and labelled at specific sites. The proteins were then reconstituted into oriented phospholipid bilayers and the resulting samples analysed. The data suggest a model in which the protein adopts a tilted helix with an angle of approximate to 30 degrees and an N-terminal 'swinging arm' at the membrane surface.

机译：丝状噬菌体的外壳蛋白首先合成为跨膜蛋白，然后组装到挤出的病毒颗粒上。我们使用质子去耦的N-15和P-31固态NMR光谱研究了铜绿假单胞菌Pf3噬菌体外壳蛋白的跨膜构象。蛋白质经过生物化学纯化并用N-15均匀标记，或化学合成并在特定位点标记。然后将蛋白质重构为定向的磷脂双层，并分析所得的样品。数据提示了一种模型，其中蛋白质采用倾斜的螺旋结构，其角度约为30度，并在膜表面形成N端“摆动臂”。

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《The FEBS journal》 |2006年第4期|共12页
作者
Aisenbrey C; Harzer U; Bauer-Manz G; Bar G; Chotimah INH; Bertani P; Sizun C; Kuhn A; Bechinger B;
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正文语种 eng
中图分类生物化学;
关键词
oriented lipid bilayer; amphipathic alpha helix; membrane protein structure; protein dynamics; mosaic spread; MEMBRANE-PROTEIN; M13 COAT; DETERGENT MICELLES; BACTERIOPHAGE M13; SPECTROSCOPY; INSERTION; PEPTIDES; DYNAMICS; ACID; ALIGNMENT;

机译：定向脂质双层;两亲性α螺旋;膜蛋白结构;蛋白动力学;马赛克;膜蛋白;M13外套;洗涤剂胶束;细菌噬菌体M13;光谱学;插入;肽;动力学;酸;对准;

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1. Proton-decoupled N-15 and P-31 solid-state NMR investigations of the Pf3 coat protein in oriented phospholipid bilayers [J] . Aisenbrey C, Harzer U, Bauer-Manz G, The FEBS journal . 2006,第4期

机译：定向磷脂双层中Pf3外壳蛋白的质子解耦N-15和P-31固态NMR研究
2. Proton-decoupled 15N and 31P solid-state NMR investigations of the Pf3 coat protein in oriented phospholipid bilayers [J] . Aisenbrey C, Harzer U, Bauer-Manz G, The FEBS journal . 2006,第4期

机译：定向磷脂双层中Pf3外壳蛋白的质子解耦15N和31P固态NMR研究
3. Proton-decoupled 15N and 31P solid-state NMR investigations of the Pf3 coat protein in oriented phospholipid bilayers [J] . Aisenbrey C, Harzer U, Bauer-Manz G, The FEBS journal . 2006,第4期

机译：定向磷脂双层中Pf3外壳蛋白的质子解耦15N和31P固态NMR研究
4. Mutant coat proteins of Pf3 bacteriophage as models of membrane proteins and their interactions with lipid bilayer membrane [C] . Taisuke Matsuo, Naoshi Yamazaki, Tatsuhiro Ishida, IEEE International Symposium on Micro-NanoMechatronics and Human Science . 2006

机译：PF3噬菌体的突变涂层蛋白作为膜蛋白的模型及其与脂质双层膜的相互作用
5. NMR investigations of blood coagulation: Conformational changes of membrane bilayers and proteins in blood coagulation. [D] . Boettcher, John Micheal. 2010

机译：血液凝固的NMR研究：血液凝固中膜双层和蛋白质的构象变化。
6. Improved 1H amide resonance line narrowing in oriented sample solid-state NMR of membrane proteins in phospholipid bilayers [O] . George J. Lu, Sang Ho Park, Stanley J. Opella -1

机译：改进的磷脂双层膜蛋白的取向样品固态NMR缩小的1H酰胺共振线
7. Proton-decoupled 15N and 31P solid-state NMR investigations of the Pf3 coat protein in oriented phospholipid bilayers [O] . Christopher Aisenbrey, Ulrike Harzer, Gabriele Bauer-Manz, 2006

机译：PF3涂层蛋白在取向磷脂双层的PF3涂层蛋白的质子去耦15N和31P固态NMR研究

Proton-decoupled N-15 and P-31 solid-state NMR investigations of the Pf3 coat protein in oriented phospholipid bilayers

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