...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The FEBS journal >Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs
【24h】

Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs

机译：具有非经典活性中心基序的大豆蛋白二硫键异构酶家族蛋白的分子克隆和表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Protein disulfide isomerase (PDI) and other PDI family proteins are members of the thioredoxin superfamily and are thought to play important roles in disulfide bond formation and isomerization in the endoplasmic reticulum (ER). The exact functions of PDI family proteins in plants remain unknown. In this study, we cloned two novel PDI family genes from soybean leaf (Glycine max L. Merrill cv. Jack). The cDNAs encode proteins of 520 and 523 amino acids, and have been denoted GmPDIL-3a and GmPDIL-3b, respectively. GmPDIL-3a and GmPDIL-3b are the first plant ER PDI family proteins reported to contain the nonclassic redox center motif CXXS/C, and both proteins are ubiquitously expressed in the plant body. However, recombinant GmPDIL-3a and GmPDIL-3b did not function as oxidoreductases or as molecular chaperones in vitro, although a proportion of each protein formed complexes in both thiol-dependent and thiol-independent ways in the ER. Expression of GmPDIL-3a and GmPDIL-3b in the cotyledon increased during seed maturation when synthesis of storage proteins was initiated. These results suggest that GmPDIL-3a and GmPDIL-3b may play important roles in the maturation of the cotyledon by mechanisms distinct from those of other PDI family proteins. Structured digital abstract MINT-7137566: Bip (uniprotkb: Q587K1), GmPDIL-3b (genbank_nucleotide_g: 51848586) and GmPDIL-3a (genbank_nucleotide_g: 51848584) colocalize ( MI:0403) by cosedimentation through density gradients ( MI:0029)
机译：蛋白质二硫键异构酶（PDI）和其他PDI家族蛋白是硫氧还蛋白超家族的成员，被认为在内质网（ER）的二硫键形成和异构化中起重要作用。 PDI家族蛋白在植物中的确切功能仍然未知。在这项研究中，我们从大豆叶片（Glycine max L. Merrill cv。Jack）中克隆了两个新的PDI家族基因。 cDNA编码520和523个氨基酸的蛋白质，分别表示为GmPDIL-3a和GmPDIL-3b。 GmPDIL-3a和GmPDIL-3b是第一个据报道含有非经典氧化还原中心基序CXXS / C的植物ER PDI家族蛋白，两种蛋白均在植物体内普遍表达。然而，尽管在ER中，每种蛋白质的一部分以硫醇依赖性和硫醇依赖性方式形成复合物，但是重组GmPDIL-3a和GmPDIL-3b在体外没有作为氧化还原酶或分子伴侣的功能。启动存储蛋白合成时，种子成熟期间子叶中GmPDIL-3a和GmPDIL-3b的表达增加。这些结果表明，GmPDIL-3a和GmPDIL-3b可能通过不同于其他PDI家族蛋白的机制在子叶成熟中发挥重要作用。结构化数字摘要MINT-7137566：Bip（uniprotkb：Q587K1），GmPDIL-3b（genbank_nucleotide_g：51848586）和GmPDIL-3a（genbank_nucleotide_g：51848584）通过密度梯度的共沉降（MI：0029）共定位（MI：0403）

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号