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首页> 外文期刊>The FEBS journal >Conservation of the egg envelope digestion mechanism of hatching enzyme in euteleostean fishes
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Conservation of the egg envelope digestion mechanism of hatching enzyme in euteleostean fishes

机译：真鱼的孵化酶卵壳消化机制的保护

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摘要

We purified two hatching enzymes, namely high choriolytic enzyme (HCE; EC 3.4.24.67) and low choriolytic enzyme (LCE; EC 3.4.24.66), from the hatching liquid of Fundulus heteroclitus, which were named Fundulus HCE (FHCE) and Fundulus LCE (FLCE). FHCE swelled the inner layer of egg envelope, and FLCE completely digested the FHCE-swollen envelope. In addition, we cloned three Fundulus cDNAs orthologous to cDNAs for the medaka precursors of egg envelope subunit proteins (i.e. choriogenins H, H minor and L) from the female liver. Cleavage sites of FHCE and FLCE on egg envelope subunit proteins were determined by comparing the N-terminal amino acid sequences of digests with the sequences deduced from the cDNAs for egg envelope subunit proteins. FHCE and FLCE cleaved different sites of the subunit proteins. FHCE efficiently cleaved the Pro-X-Y repeat regions into tripeptides to dodecapeptides to swell the envelope, whereas FLCE cleaved the inside of the zona pellucida domain, the core structure of egg envelope subunit protein, to completely digest the FHCE-swollen envelope. A comparison showed that the positions of hatching enzyme cleavage sites on egg envelope subunit proteins were strictly conserved between Fundulus and medaka. Finally, we extended such a comparison to three other euteleosts (i.e. three-spined stickleback, spotted halibut and rainbow trout) and found that the egg envelope digestion mechanism was well conserved among them. During evolution, the egg envelope digestion by HCE and LCE orthologs was established in the lineage of euteleosts, and the mechanism is suggested to be conserved.
机译：我们从Fund藜的孵化液中纯化了两种孵化酶，分别是高绒毛分解酶（HCE; EC 3.4.24.67）和低绒毛分解酶（LCE; EC 3.4.24.66），分别称为Fund木HCE（FHCE）和Fund木LCE （FLCE）。 FHCE使卵壳的内层膨胀，而FLCE完全消化了FHCE肿胀的壳。此外，我们从雌性肝脏中克隆了三个与卵壳蛋白亚基蛋白（即胆囊生成素H，H和H，H和L）的medaka前体同源的cDNAcDNA。通过将消化物的N-末端氨基酸序列与从蛋包膜亚基蛋白的cDNA推导出的序列进行比较，确定了蛋包膜亚基蛋白上的FHCE和FLCE的切割位点。 FHCE和FLCE切割了亚基蛋白的不同位点。 FHCE有效地将Pro-X-Y重复区切割成三肽以使十二肽溶胀包膜，而FLCE切割了卵包膜亚基蛋白的核心结构透明带结构域的内部，以完全消化FHCE肿胀的包膜。比较表明，在卵壳和青aka之间，卵包膜亚基蛋白上的孵化酶切割位点的位置是严格保守的。最后，我们将这种比较扩展到了另外三个雌性硬皮动物（即三棘刺背，斑点大比目鱼和虹鳟鱼），发现其中的卵壳消化机制非常保守。在进化过程中，通过HCE和LCE直系同源物消化的卵被膜被建立在真骨动物的血统中，并且该机制被认为是保守的。

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