...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The FEBS journal >Calcium-independent phospholipase A-mediated formation of 1,2-diarachidonoyl-glycerophosphoinositol in monocytes
【24h】

Calcium-independent phospholipase A-mediated formation of 1,2-diarachidonoyl-glycerophosphoinositol in monocytes

机译：钙非依赖性磷脂酶A介导的单核细胞中1,2-二花生四烯酰基-甘油磷酸肌醇的形成

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Phagocytic cells exposed to exogenous arachidonic acid (AA) incorporate large quantities of this fatty acid into choline and ethanolamine glycerophospholipids, and into phosphatidylinositol (PtdIns). Utilizing liquid chromatography coupled to MS, we have characterized the incorporation of exogenous deuterated AA ([(2)H]AA) into specific PtdIns molecular species in human monocyte cells. A PtdIns species containing two exogenous [(2)H]AA molecules (1-[(2)H]AA-2-[(2)H]AA-glycero-3-phosphoinositol) was readily detected when human U937 monocyte-like cells and peripheral blood monocytes were exposed to [(2)H]AA concentrations as low as 160 nm to 1 mum. Bromoenol lactone, an inhibitor of Ca(2+)-independent phospholipase A(2) (iPLA(2)), diminished lyso-PtdIns levels, and almost completely inhibited the appearance of 1-[(2)H]AA-2-[(2)H]AA-glycero-3-phosphoinositol, suggesting the involvement of deacylation reactions in the synthesis of this phospholipid. De novo synthesis did not appear to be involved, as no other diarachidonoyl phospholipid or neutral lipid was detected under these conditions. Measurement of the metabolic fate of 1-[(2)H]AA-2-[(2)H]AA-glycero-3-phosphoinositol after pulse-labeling of the cells with [(2)H]AA showed a time-dependent, exponential decrease in the level of this phospholipid. These results identify 1-[(2)H]AA-2-[(2)H]AA-glycero-3-phosphoinositol as a novel, short-lived species for the initial incorporation of AA into the PtdIns class of cellular phospholipids in human monocytes.
机译：暴露于外源花生四烯酸（AA）的吞噬细胞将大量这种脂肪酸掺入胆碱和乙醇胺甘油磷脂中，并掺入磷脂酰肌醇（PtdIns）中。利用与MS耦合的液相色谱，我们表征了将外源氘代AA（[（2）H] AA）掺入人单核细胞中特定的PtdIns分子物种中。当人U937单核细胞样的时候很容易检测到包含两个外源[[2）H] AA分子（1-[（2）H] AA-2-[（2）H] AA-甘油-3-磷酸肌醇）的PtdIns物种。细胞和外周血单核细胞暴露于[（2）H] AA浓度低至160纳米至1毫米。溴烯醇内酯，Ca（2+）独立的磷脂酶A（2）（iPLA（2））的抑制剂，减少了溶血素PtdIns的水平，几乎完全抑制了1-[（（2）H] AA-2- [（2）H] AA-甘油-3-磷酸肌醇，表明脱脂反应参与了该磷脂的合成。从头合成似乎未涉及，因为在这些条件下未检测到其他二花生四烯酰基磷脂或中性脂质。用[（2）H] AA脉冲标记细胞后，测量1-[（（2）H] AA-2-[（2）H] AA-甘油-3-磷酸肌醇的代谢命运。磷脂水平的依赖性，指数下降。这些结果确定1-[（（2）H] AA-2-[（2）H] AA-甘油-3-磷酸肌醇是一种新的短命物种，用于将AA初始掺入PtdIns类细胞磷脂中。人单核细胞。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号