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首页> 外文期刊>The FEBS journal >The ORF slr0091 of Synechocystis sp. PCC6803 encodes a high-light induced aldehyde dehydrogenase converting apocarotenals and alkanals.
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The ORF slr0091 of Synechocystis sp. PCC6803 encodes a high-light induced aldehyde dehydrogenase converting apocarotenals and alkanals.

机译：蓝藻的ORF slr0091。 PCC6803编码高光诱导的醛脱氢酶转化的生物分解醛和链烷醛。

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Oxidative cleavage of carotenoids and peroxidation of lipids lead to apocarotenals and aliphatic aldehydes called alkanals, which react with vitally important compounds, promoting cytotoxicity. Although many enzymes have been reported to deactivate alkanals by converting them into fatty acids, little is known about the mechanisms used to detoxify apocarotenals or the enzymes acting on them. Cyanobacteria and other photosynthetic organisms must cope with both classes of aldehydes. Here we report that the Synechocystis enzyme SynAlh1, encoded by the ORF slr0091, is an aldehyde dehydrogenase that mediates oxidation of both apocarotenals and alkanals into the corresponding acids. Using a crude lysate of SynAlh1-expressing Escherichia coli cells, we show that SynAlh1 converts a wide range of apocarotenals and alkanals, with a preference for apocarotenals with defined chain lengths. As suggested by in vitro incubations and using engineered retinal-forming E. coli cells, we found that retinal is not a substrate for SynAlh1, making involvement in Synechocystis retinoid metabolism unlikely. The transcript level of SynAlh1 is induced by high light and cold treatment, indicating a role in the stress response, and the corresponding gene is a constituent of a stress-related operon. The assumptions regarding the function of SynAlh are further supported by the surprisingly high homology to human and plant aldehyde dehydrogenase that have been assigned to aldehyde detoxification. SynAlh1 is the first aldehyde dehydrogenase that has been shown to form both apocarotenoic and fatty acids. This dual function suggests that its eukaryotic homologs may also be involved in apocarotenal metabolism, a function that has not been considered so far. 2013 FEBS.Registry Number/Name of Substance 0 (Aldehydes). 0 (Alkanes). 0 (Bacterial Proteins). 1107-26-2 (apocarotenal). 36-88-4 (Carotenoids). EC 1-2-1-3 (Aldehyde Dehydrogenase).
机译：类胡萝卜素的氧化裂解和脂质的过氧化作用导致被称为链烷醛的类胡萝卜素和脂肪醛与重要的化合物反应，从而促进细胞毒性。尽管已经报道了许多酶通过将链烷烃转化为脂肪酸来使其失活，但是人们对用于解附质的解毒作用机理或作用于它们的酶知之甚少。蓝细菌和其他光合作用生物必须应对这两类醛。在这里，我们报告说，由ORF slr0091编码的集囊藻类酶SynAlh1是一种醛脱氢酶，它介导了细胞分裂和链烷醛氧化成相应的酸。使用表达SynAlh1的大肠杆菌细胞的粗裂解液，我们显示SynAlh1可以转换多种载脂细胞和链烷醛，偏爱具有定义链长的载脂细胞。正如体外培养和使用工程化的视网膜形成大肠杆菌细胞所暗示的那样，我们发现视网膜不是SynAlh1的底物，因此不可能参与集胞囊藻类维生素A的代谢。 SynAlh1的转录水平是由强光和冷处理诱导的，表明其在应激反应中起作用，并且相应的基因是应激相关操纵子的组成部分。关于SynAlh功能的假设进一步得到了与人和植物醛脱氢酶高度同源的惊人支持，这些同源性已被赋予醛解毒作用。 SynAlh1是第一种醛脱氢酶，已被证明能同时形成载脂蛋白和脂肪酸。这种双重功能表明其真核同源物也可能参与了非肢体细胞的新陈代谢，这一功能至今尚未被考虑。 2013年FEBS。注册号/物质名称0（醛）。 0（烷烃）。 0（细菌蛋白）。 1107-26-2（末日）。 36-88-4（类胡萝卜素）。 EC 1-2-1-3（醛脱氢酶）。

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