...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bulletin of the Chemical Society of Japan >Characterization of the Peroxidase Activity of Single Algae Protoplasts by Scanning Electrochemical Microscopy
【24h】

Characterization of the Peroxidase Activity of Single Algae Protoplasts by Scanning Electrochemical Microscopy

机译：扫描电化学显微镜表征单个藻原生质体的过氧化物酶活性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The peroxidase activity in a single protoplast of alga Bropsis plumosa is quantitatively characterized by scanning electrochemical microscopy.The generationof ferriceniummethanol(FMA~+)at the protoplast surface is directly detected by the microelectrode tip scanned close to the sample surface in seawater containing ferrocenemethanol (FMA)and hdyrogen peroxide,an electron mediator and an enzyme-substrate,respectily.The oxidation reaction requires hydrogen peroxide,which clearly shows FMA~+ generation due to the peroxidase (POD)catalytic reaction occurring in the protoplast.The FMA~+ generation and the FMA accumultion rates at a single alga protoplast were equivalent.A plot of the FMA~+ generation rates according to the hydrogen peroxide concentrations was well allowed with a Michaelis-Menten-type reaction.An estimation of the masstrnasfer rate and a determination of the K_m are quite important advantages of the SECM technique that cannot be realized using other techniques.The POD activity has been further investigated from the viewpoint of the size of the protoplast.The POD activity of the alga in the adult stage is also visualized by SECM.The noninvasive nature of the SECM technique has been confirmed by observing the developmental process after measurements.
机译：通过扫描电化学显微镜对藻类单生藻的单个原生质体中的过氧化物酶活性进行定量表征。通过在含二茂铁甲醇（FMA）的海水中靠近样品表面扫描的微电极尖端直接检测原生质体表面甲醇ethanol（FMA〜+）的生成。氧化反应需要过氧化氢，这清楚地表明FMA〜+的生成是由于原生质体中发生了过氧化物酶（POD）催化反应。一个藻类原生质体的FMA累积速率是等价的。通过Michaelis-Menten型反应很好地绘制了根据过氧化氢浓度的FMA〜+生成速率图。 K_m是SECM技术的相当重要的优势，而其他技术则无法实现。从原生质体的大小角度进一步研究了该类型.SECM还可以观察成年期藻类的POD活性。通过测量后观察其发育过程，可以确认SECM技术的无创性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号