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首页> 外文期刊>Bulletin of the Chemical Society of Japan >Selective Detection of Human Serum Albumin Using a Fused-Silica Capillary Modified with Anti-Human Serum Albumin
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Selective Detection of Human Serum Albumin Using a Fused-Silica Capillary Modified with Anti-Human Serum Albumin

机译:使用抗人血清白蛋白修饰的熔融石英毛细管选择性检测人血清白蛋白

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摘要

A fused-silica capillary modified with anti-human serum albumin was prepared using the glutaraldehyde method after activating the inner wall with a silane coupling reagent.The antibody selectively interacted via an immune reaction with the human serum albumin,which acted as an antigen.The analytical performance of the modified capillary was examined by capillary affinity chromatography using either absorption or chemiluminescence detection.The capillary affinity chromatography required only minimal reagents and samples due to the extremely small diameter of the capillary.For chemiluminescence detection,a peroxyoxalate reagent was used and human serum albumin was labeled with fluoresc-amine for competitive immunoassay.After optimization of the analytical conditions,chemiluminescence detection showed a higher sensitivity than absorption detection.Furthermore,a modified capillary was also used in capillary elec-trophoresis with the absorption detector to allow affinity capillary electrophoresis.
机译:用硅烷偶联剂活化内壁后,用戊二醛法制备了抗人血清白蛋白修饰的石英毛细管,该抗体通过免疫反应与作为抗原的人血清白蛋白选择性相互作用。毛细管亲和色谱采用吸收或化学发光检测法检测改性毛细管的分析性能。由于毛细管的直径极小,毛细管亲和色谱仅需最少的试剂和样品。化学发光检测使用过氧草酸酯试剂和人血清白蛋白用荧光胺标记,用于竞争性免疫分析。优化分析条件后,化学发光检测显示出比吸收检测更高的灵敏度。此外,修饰的毛细管也用于毛细管电泳,并带有吸收检测器以允许亲和毛细管电泳矿石。

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