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首页> 外文期刊>Biomaterials Science >Non-monotonic cell differentiation pattern on extreme wettability gradients
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Non-monotonic cell differentiation pattern on extreme wettability gradients

机译：极端润湿性梯度上的非单调细胞分化模式

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In this study, we propose a methodology to obtain a family of biomimetic substrates with a hierarchical rough topography at the micro and nanoscale that span the entire range of wettability, from the super-hydrophobic to the superhydrophilic regime, through an Ar-plasma treatment at increasing durations. Moreover, we employ the same approach to produce a superhydrophobic-to-superhydrophilic surface gradient along centimetre-length scale distances within the same sample. We characterize the biological activity of these surfaces in terms of protein adsorption and cell response, using fibronectin, a major component of the extracellular matrix, and C2C12 cells, a myoblast cell line. Fibronectin conformation, assessed via binding of the monoclonal antibody HFN7.1, exhibits a non-monotonic dependence on surface wettability, with higher activity on hydrophilic substrates (WCA = 38.6 ± 8.1°). On the other hand, the exposition of cell-binding epitopes is diminished on the surfaces with extreme wetting properties, the conformation being particularly altered on the superhydrophobic substrate. The assessment of cell response via the myogenic differentiation process reveals that a gradient surface promotes a different response with respect to cells cultured on discrete uniform samples: even though in both cases the same non-monotonic differentiation pattern is found, the differential response to the various wettabilities is enhanced along the gradient while the overall levels of differentiation are diminished. On a gradient surface cells are in fact exposed to a range of continuously changing stimuli that foster cell migration and detain the differentiation process.
机译：在这项研究中，我们提出了一种方法，该方法可通过在室温下进行Ar-等离子体处理，在微观和纳米尺度上获得具有仿生粗糙表面形貌的仿生底物家族，这些仿生底物覆盖整个润湿性范围，从超疏水性到超亲水性。持续时间增加。此外，我们采用相同的方法在同一样品内沿厘米长度尺度距离生成超疏水性至超亲水性表面梯度。我们使用纤维连接蛋白（一种细胞外基质的主要成分）和C2C12细胞（一种成肌细胞系），根据蛋白质吸附和细胞反应来表征这些表面的生物学活性。通过结合单克隆抗体HFN7.1评估的纤连蛋白构象对表面润湿性表现出非单调依赖性，对亲水性底物具有更高的活性（WCA = 38.6±8.1°）。另一方面，细胞结合表位的暴露在具有极端润湿性质的表面上减少，该构象在超疏水性底物上特别改变。通过成肌分化过程对细胞反应的评估表明，相对于在离散的均匀样本上培养的细胞，梯度表面促进了不同的反应：即使在两种情况下都发现了相同的非单调分化模式，对各种细胞的分化反应沿梯度增加了润湿性，而总体分化水平却降低了。实际上，在梯度表面上，细胞会受到一系列不断变化的刺激，从而促进细胞迁移并保持分化过程。

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