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首页> 外文期刊>Bulletin of the Chemical Society of Japan >Assembly of Methylene Blue on Nucleic Acid Template as Studied by Resonance Light-Scattering Technique and Determination of Nucleic Acids of Nanogram
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Assembly of Methylene Blue on Nucleic Acid Template as Studied by Resonance Light-Scattering Technique and Determination of Nucleic Acids of Nanogram

机译：共振光散射技术研究亚甲蓝在核酸模板上的组装及核酸的测定

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摘要

The template role nucleic acids in the long-range assembly of Methylene BLue (MB) was characterized with Resonance Light-Scattering (RLS) measurements, and a determination method of nucleic acids of nanogram was proposed. At the pH range 6.87-8.74 and ionic strength lower than 0.01, the interactions of MB with nucleic acids result in two characteristic RLS peaks at 355.0 and 560.0 nm. Mechanism studies show that these peaks result from the long-range assembly of MB on the molecular surface of nucleic acids. A Scatchard plot was constructed by using the RLS data, yielding an assembly number and assembly constant of 5.67 and 9.22 * 10~4 mol~(-1) dim~3 for MB assembly on the molecular surface of calf thymus DNA (ctDNA), 5.79 and 4.68 * 10~4 mol~(-1) dm~3 for MB assembly on that of fish sperm DNA (fsDNA). This assembly could be used for analytical purposes and 0-1.4 #mu#g ml~(-1) for ctDNA, 0-1.2 #mu#g ml~(-1) for fsDNA, and 0-0.24 #mu#g ml~(-1) for yeast RNA could be determined if 3.0 * 10~(-5) mol dm~(-3) MB was employed. The limits of determination were 11.0 ng ml~(-1) for ctDNA, 4.9 ng ml~(-1) for fsDNA and 8.6 ng ml~(-1) for yeast RNA, respectively, Four synthetic samples were determined with satisfaction.
机译：通过共振光散射（RLS）测量对亚甲基蓝（MB）长距离组装中的模板作用核酸进行了表征，提出了纳克核酸的测定方法。在pH范围6.87-8.74和离子强度低于0.01时，MB与核酸的相互作用导致在355.0和560.0 nm处出现两个特征性RLS峰。机理研究表明，这些峰是由于MB在核酸分子表面上的长距离组装而产生的。利用RLS数据构建了Scatchard图，得出小牛胸腺DNA（ctDNA）分子表面MB的组装数和组装常数分别为5.67和9.22 * 10〜4 mol〜（-1）dim〜3。 5.79和4.68 * 10〜4 mol〜（-1）dm〜3用于MB组装在鱼精DNA（fsDNA）上。该装配体可用于分析目的，ctDNA可使用0-1.4＃mu＃g ml〜（-1），fsDNA可使用0-1.2＃mu＃g ml〜（-1），0-0.24＃mu＃g ml如果使用3.0 * 10〜（-5）mol dm〜（-3）MB，则可以确定酵母RNA的〜（-1）。 ctDNA的测定极​​限分别为11.0 ng ml〜（-1），fsDNA的测定极​​限为4.9 ng ml〜（-1），酵母RNA的测定极​​限为8.6 ng ml〜（-1），满意地测定了四个合成样品。

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