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首页> 外文期刊>Bulletin of the Chemical Society of Japan >Calf-Thymus DNA Interaction with Cr(III)-Gallate and Cr(III)-Ethyl Gallate Studied by FTIR Spectroscopy and Capillary Electrophoresis
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Calf-Thymus DNA Interaction with Cr(III)-Gallate and Cr(III)-Ethyl Gallate Studied by FTIR Spectroscopy and Capillary Electrophoresis

机译：FTIR光谱和毛细管电泳研究小牛胸腺DNA与加仑酸Cr（III）和没食子酸Cr（III）的相互作用

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摘要

Cr(VI) salts are well known to be carcinogen, and are reduced by various cellular components to form cross-linked Cr(III) products. Organic compounds, such as gallic acid (GA) and ethyl gallate (EGA), reduce Cr(VI) to Cr(III) to form Cr(III)-gallate [Cr(III)-GA] and Cr(III)-ethyl gallate [Cr(III)-EGA] as final products. These Cr(III)-tannin complexes are DNA binders. The interaction of calf-thymus DNA with Cr(III)-GA and Cr(III)-EGA in aqueous solutions at physiological pH were studied at Cr(III)/DNA (phosphate) molar ratios (r) of 1:160, 1:80, 1:40, 1:20, 1:10, 1:4, and 1:2 using FTIR spectroscopy and capillary electrophoresis. An analysis by FTIR showed that at low concentrations (r = 1/80 and 1/40), Cr(III)-GA and Cr(III)-EGA mainly bind to the guanine N-7 atom of the G-C base pairs with minor perturbations of the A-T base pairs. At r > 1/20, a partial helix opening occurred with major perturbations of the G-C and A-T base pairs. At r >1/10, aggregation of the Cr(III)-tannin-DNA complexes occurred. However, no DNA conformational changes were observed, and the DNA maintained the B-farnily structure. The binding constants of the Cr(III)-GA-DNA and Cr(III)-EGA-DNA were estimated to be 3.8 * 10~4 M~(-1) and 6.2*10~4 M~(-1), respectively, by Scatchard plots following capillary electrophoresis. These results suggest that the Cr(III)-tannin complexes are external DNA binders, and do not form a chelate with the DNA~.
机译：众所周知，Cr（VI）盐是致癌物，并被各种细胞成分还原而形成交联的Cr（III）产物。有机化合物，例如没食子酸（GA）和没食子酸乙酯（EGA），将Cr（VI）还原为Cr（III），形成Cr（III）-没食子酸酯[Cr（III）-GA]和Cr（III）-乙基没食子酸酯[Cr（III）-EGA]作为终产物。这些Cr（III）-鞣质复合物是DNA结合剂。在Cr（III）/ DNA（磷酸盐）摩尔比（r）为1：160，1的条件下，研究了小牛胸腺DNA与Cr（III）-GA和Cr（III）-EGA在水溶液中的相互作用。使用FTIR光谱学和毛细管电泳分析：80、1：40、1：20、1：10、1：4和1：2。 FTIR分析表明，在低浓度（r = 1/80和1/40）下，Cr（III）-GA和Cr（III）-EGA主要与GC碱基对的鸟嘌呤N-7原子结合，而次要AT碱基对的扰动。在r> 1/20时，发生部分螺旋开口，并出现了G-C和A-T碱基对的主要扰动。在r> 1/10时，发生Cr（III）-单宁-DNA复合物的聚集。但是，没有观察到DNA构象变化，并且DNA保持了B型结构。 Cr（III）-GA-DNA和Cr（III）-EGA-DNA的结合常数估计为3.8 * 10〜4 M〜（-1）和6.2 * 10〜4 M〜（-1），分别通过毛细管电泳后的Scatchard图绘制。这些结果表明Cr（III）-鞣质复合物是外部DNA结合剂，并且不与DNA形成螯合物。

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